Literature DB >> 8063094

Gene sequence, purification and characterization of N-acetyl-beta-glucosaminidase from a marine bacterium, Alteromonas sp. strain O-7.

H Tsujibo1, K Fujimoto, H Tanno, K Miyamoto, C Imada, Y Okami, Y Inamori.   

Abstract

The gene (cht60) encoding N-acetyl-beta-glucosaminidase (Cht; EC 3.2.1.30) from the marine bacterium Alteromonas sp. strain O-7 was cloned into pUC18 in Escherichia coli JM109. The nucleotide (nt) sequence of cht60 was determined. A 1797-bp open reading frame encoded a polypeptide of 598 amino acids (aa) (M(r) 64,535). The aa sequence of the cloned enzyme (Cht60) deduced from the nt sequence showed no significant sequence homologies with available aa sequences from databases. Cht60 was purified from the periplasmic fraction of E. coli cells carrying pCHT982. The enzyme was most active towards p-nitrophenyl-N-acetyl-beta-D-glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose. The optimum pH and temperature of the enzyme were pH 7.5 and 37 degrees C, respectively. The N-terminal 11 aa residues of Cht60 were sequenced, and the location of the signal peptide cleavage site was clarified.

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Year:  1994        PMID： 8063094     DOI： 10.1016/0378-1119(94)90843-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  15 in total

Review 1.  The family-3 glycoside hydrolases: from housekeeping functions to host-microbe interactions.

Authors:  Denis Faure
Journal:  Appl Environ Microbiol       Date:  2002-04       Impact factor: 4.792

2.  Characterization of chitinase C from a marine bacterium, Alteromonas sp. strain O-7, and its corresponding gene and domain structure.

Authors:  H Tsujibo; H Orikoshi; K Shiotani; M Hayashi; J Umeda; K Miyamoto; C Imada; Y Okami; Y Inamori
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

3.  Molecular analysis of the gene encoding a novel transglycosylative enzyme from Alteromonas sp. strain O-7 and its physiological role in the chitinolytic system.

Authors:  H Tsujibo; N Kondo; K Tanaka; K Miyamoto; N Baba; Y Inamori
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

4.  Identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, Alteromonas sp. strain O-7.

Authors:  Hiroshi Tsujibo; Hideyuki Orikoshi; Nao Baba; Masahiro Miyahara; Katsushiro Miyamoto; Masahide Yasuda; Yoshihiko Inamori
Journal:  Appl Environ Microbiol       Date:  2002-01       Impact factor: 4.792

5.  Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis.

Authors:  Mark J McBride; Gary Xie; Eric C Martens; Alla Lapidus; Bernard Henrissat; Ryan G Rhodes; Eugene Goltsman; Wei Wang; Jian Xu; David W Hunnicutt; Andrew M Staroscik; Timothy R Hoover; Yi-Qiang Cheng; Jennifer L Stein
Journal:  Appl Environ Microbiol       Date:  2009-08-28       Impact factor: 4.792

6.  A novel beta-N-acetylglucosaminidase from Streptomyces thermoviolaceus OPC-520: gene cloning, expression, and assignment to family 3 of the glycosyl hydrolases.

Authors:  H Tsujibo; N Hatano; T Mikami; A Hirasawa; K Miyamoto; Y Inamori
Journal:  Appl Environ Microbiol       Date:  1998-08       Impact factor: 4.792

7.  Molecular cloning of the gene which encodes beta-N-acetylglucosaminidase from a marine bacterium, Alteromonas sp. strain O-7.

Authors:  H Tsujibo; K Fujimoto; H Tanno; K Miyamoto; Y Kimura; C Imada; Y Okami; Y Inamori
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

8.  Roles of four chitinases (chia, chib, chic, and chid) in the chitin degradation system of marine bacterium Alteromonas sp. strain O-7.

Authors:  Hideyuki Orikoshi; Shigenari Nakayama; Katsushiro Miyamoto; Chiaki Hanato; Masahide Yasuda; Yoshihiko Inamori; Hiroshi Tsujibo
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

9.  Molecular analysis of the gene encoding a novel chitin-binding protease from Alteromonas sp. strain O-7 and its role in the chitinolytic system.

Authors:  Katsushiro Miyamoto; Eiji Nukui; Hiroyuki Itoh; Takaji Sato; Takeshi Kobayashi; Chiaki Imada; Etsuo Watanabe; Yoshihiko Inamori; Hiroshi Tsujibo
Journal:  J Bacteriol       Date:  2002-04       Impact factor: 3.490

10.  Flanking signal and mature peptide residues influence signal peptide cleavage.

Authors:  Khar Heng Choo; Shoba Ranganathan
Journal:  BMC Bioinformatics       Date:  2008-12-12       Impact factor: 3.169
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