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Literature DB >> 8060991

Mapping RNA regions in eukaryotic ribosomes that are accessible to methidiumpropyl-EDTA.Fe(II) and EDTA.Fe(II).

H Han¹, A Schepartz, M Pellegrini, P B Dervan.

Abstract

Methidiumpropyl-EDTA.Fe(II) [MPE.Fe(II)] and EDTA.Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE.Fe(II) and EDTA.Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.

Entities: Chemical Disease Species

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Year: 1994 PMID： 8060991 DOI： 10.1021/bi00199a004

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

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Cited

11 in total

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2. Diffusion-based transport of nascent ribosomes in the nucleus.

Authors: Joan C Ritland Politz; Richard A Tuft; Thoru Pederson
Journal: Mol Biol Cell Date: 2003-09-05 Impact factor: 4.138

3. Cleavage of rRNA ensures translational cessation in sperm at fertilization.

Authors: G D Johnson; E Sendler; C Lalancette; R Hauser; M P Diamond; S A Krawetz
Journal: Mol Hum Reprod Date: 2011-08-10 Impact factor: 4.025

4. Potent antisense oligonucleotides to the human multidrug resistance-1 mRNA are rationally selected by mapping RNA-accessible sites with oligonucleotide libraries.

Authors: S P Ho; D H Britton; B A Stone; D L Behrens; L M Leffet; F W Hobbs; J A Miller; G L Trainor
Journal: Nucleic Acids Res Date: 1996-05-15 Impact factor: 16.971

Mapping RNA regions in eukaryotic ribosomes that are accessible to methidiumpropyl-EDTA.Fe(II) and EDTA.Fe(II).

1. Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction.

2. Diffusion-based transport of nascent ribosomes in the nucleus.

3. Cleavage of rRNA ensures translational cessation in sperm at fertilization.

4. Potent antisense oligonucleotides to the human multidrug resistance-1 mRNA are rationally selected by mapping RNA-accessible sites with oligonucleotide libraries.

Review 5. Divergence towards a dead end? Cleavage of the divergent domains of ribosomal RNA in apoptosis.

6. Fine mapping of 28S rRNA sites specifically cleaved in cells undergoing apoptosis.

7. Novel Biochemical Tools for Probing HIV RNA Structure.

8. Antisense ribosomes: rRNA as a vehicle for antisense RNAs.

9. rRNA expansion segment 7 in eukaryotes: from Signature Fold to tentacles.

10. The HIV-2 Rev-response element: determining secondary structure and defining folding intermediates.