| Literature DB >> 8060679 |
Abstract
An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4 degrees C is active in polymerase chain reaction experiments for more than a year.Entities:
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Year: 1994 PMID： 8060679 DOI： 10.1016/1050-3862(94)90003-5
Source DB: PubMed Journal: Genet Anal Tech Appl ISSN： 1050-3862