| Literature DB >> 8057914 |
Abstract
Insertion mutagenesis using TnphoA has proved to be a potent device for the creation of easily screened knockout mutations in genes encoding virulence determinants in a variety of pathogenic bacteria. Initial identification of genes with TnphoA directly initiates more sophisticated genetic and biochemical studies on these factors essential to our understanding of bacterial pathogenesis.Mesh:
Substances:
Year: 1994 PMID: 8057914 DOI: 10.1016/0076-6879(94)35159-7
Source DB: PubMed Journal: Methods Enzymol ISSN: 0076-6879 Impact factor: 1.600