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Literature DB >> 8053572

A method for the high efficiency of water-soluble carbodiimide-mediated amidation.

Abstract

Water-soluble carbodiimides are widely used for carboxyl-amine conjugation. However, extremely variable and low yields, obtained under a variety of conditions, have been a serious problem in the coupling. A simple method, optimizing various parameters of the coupling reaction, in which N-hydroxysuccinimide is included to assist the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-catalyzed amidation reaction is described. A product yield of up to 90% is routinely achieved.

Entities: Chemical

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Year: 1994 PMID： 8053572 DOI： 10.1006/abio.1994.1144

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

41 in total

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2. Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model.

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3. Effect of curing time and concentration for a chemical treatment that improves surface gliding for extrasynovial tendon grafts in vitro.

Authors: Toshikazu Tanaka; Yu-Long Sun; Chunfeng Zhao; Mark E Zobitz; Kai-Nan An; Peter C Amadio
Journal: J Biomed Mater Res A Date: 2006-12-01 Impact factor: 4.396

A method for the high efficiency of water-soluble carbodiimide-mediated amidation.

1. Investigating thiol-modification on hyaluronan via carbodiimide chemistry using response surface methodology.

2. Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model.

3. Effect of curing time and concentration for a chemical treatment that improves surface gliding for extrasynovial tendon grafts in vitro.

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