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Literature DB >> 805332

Novel interference in thiobarbituric acid assay for lipid peroxidation.

W A Baumgartner, N Baker, V A Hill, E T Wright.

Abstract

The thiobarbituric acid test for lipid peroxidation, when applied to a mixture of acetaldehyde and sucrose, produces a 532 nm aborbing chromogen which is indistinguishable from that formed by malonaldehyde and thiobarbituric acid. Unless special procedures are adopted to correct for this effect, the combined action of acetaldehyde and sucrose interferes seriously with the assay of lipid peroxidation reactions, notably those implicated in alcohol-induced liver injuries. However, this unusual thiobarbituric acid effect also can be used as a sensitive method for the detection of acetaldehyde.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1975 PMID： 805332 DOI： 10.1007/BF02532707

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

11 in total

1. THE EFFECT OF INORGANIC IRON ON THE THIOBARBITURIC ACID METHOD FOR THE DETERMINATION OF LIPID PEROXIDES.

Authors: E D WILLS
Journal: Biochim Biophys Acta Date: 1964-08-05

2. Aldehyde oxidation in rat liver mitochondria.

Authors: L Smith; L Packer
Journal: Arch Biochem Biophys Date: 1972-01 Impact factor: 4.013

3. Antioxidants, lipid peroxidation and chemical-induced liver injury.

Authors: N R Di Luzio
Journal: Fed Proc Date: 1973-08

4. Studies on the TBA test for rancidity grading. II. TBA reactivity of different aldehyde classes.

Authors: R Marcuse; L Johansson
Journal: J Am Oil Chem Soc Date: 1973-10 Impact factor: 1.849

5. No chemical evidence of hepatic lipid peroxidation in acute ethanol toxicity.

Authors: S Hashimoto; R O Recknagel
Journal: Exp Mol Pathol Date: 1968-04 Impact factor: 3.362

6. The role of lipid peroxidation and antioxidants in ethanol-induced lipid alterations.

Authors: N R Luzio
Journal: Exp Mol Pathol Date: 1968-06 Impact factor: 3.362

7. Production of chromophoric substances in the thiobarbituric acid test.

Authors: L D Saslaw; L M Corwin; V S Waravdekar
Journal: Arch Biochem Biophys Date: 1966-04 Impact factor: 4.013

8. New data on the question of lipoperoxidation in carbon tetrachloride poisoning.

Authors: R O Recknagel; A K Ghoshal
Journal: Exp Mol Pathol Date: 1966-04 Impact factor: 3.362

Review 9. Autoxidation of polyunsaturated esters in water: chemical structure and biological activity of the products.

Authors: E Schauenstein
Journal: J Lipid Res Date: 1967-09 Impact factor: 5.922

10. Water soluble products of UV-irradiated autoxidized linoleic and linolenic acids.

Authors: N Baker; L Wilson
Journal: J Lipid Res Date: 1966-05 Impact factor: 5.922

6 in total

1. The time course of malondialdehyde production following impact injury to rat spinal cord as measured by microdialysis and high pressure liquid chromatography.

Authors: H Qian; D Liu
Journal: Neurochem Res Date: 1997-10 Impact factor: 3.996