Effects of manganese on growth of Mycoplasma salivarium and Mycoplasma orale.

The growth of Mycoplasma salivarium ATCC 23064 and Mycoplasma orale ATCC 15539 was inhibited by MnCl2. The growth-inhibitory effect was much more remarkable on M. orale than M. salivarium and was much more remarkable in medium supplemented with 10% (vol/vol) horse serum (HS) than 20% (vol/vol) HS. It was suggested that isolates of Mycoplasma from the oral cavity could be roughly identified as either M. salivarium and M. orale by examination of the growth (color changes) in PPLO broth supplemented with 10% (vol/vol) HS and 0.2 mM MnCl2.