Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Existence of a molecular ruler in proteasomes suggested by analysis of degradation products.

Literature DB >> 8050567

Existence of a molecular ruler in proteasomes suggested by analysis of degradation products.

T Wenzel¹, C Eckerskorn, F Lottspeich, W Baumeister.

Abstract

Analysis of the degradation products from two proteins, the insulin B-chain and human hemoglobin, generated by archaebacterial Thermoplasma acidophilum 20 S proteasomes, revealed an unexpectedly broad specificity. In spite of the vast number of different peptides found, they fell into a rather narrow size range. This suggests that a molecular ruler exists which determines the length of the cleavage products.

Entities: Species

Mesh：

Substances：

Year: 1994 PMID： 8050567 DOI： 10.1016/0014-5793(94)00665-2

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

Keyword Cloud
Cited

40 in total

Review 1. The proteasome: a macromolecular assembly designed for controlled proteolysis.

Authors: P Zwickl; D Voges; W Baumeister
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1999-09-29 Impact factor: 6.237

2. A kinetic model of vertebrate 20S proteasome accounting for the generation of major proteolytic fragments from oligomeric peptide substrates.

Authors: H G Holzhütter; P M Kloetzel
Journal: Biophys J Date: 2000-09 Impact factor: 4.033

3. Recognition principle of the TAP transporter disclosed by combinatorial peptide libraries.

Authors: S Uebel; W Kraas; S Kienle; K H Wiesmüller; G Jung; R Tampé
Journal: Proc Natl Acad Sci U S A Date: 1997-08-19 Impact factor: 11.205

4. Regulation of Protein Degradation.

Authors: J. Callis
Journal: Plant Cell Date: 1995-07 Impact factor: 11.277

5. Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus.

Authors: M W Bauer; S H Bauer; R M Kelly
Journal: Appl Environ Microbiol Date: 1997-03 Impact factor: 4.792

6. Purification and Characterization of Two Functional Forms of Intracellular Protease PfpI from the Hyperthermophilic Archaeon Pyrococcus furiosus.

Authors: S B Halio; M W Bauer; S Mukund; M Adams; R M Kelly
Journal: Appl Environ Microbiol Date: 1997-01 Impact factor: 4.792

Existence of a molecular ruler in proteasomes suggested by analysis of degradation products.

Review 1. The proteasome: a macromolecular assembly designed for controlled proteolysis.

2. A kinetic model of vertebrate 20S proteasome accounting for the generation of major proteolytic fragments from oligomeric peptide substrates.

3. Recognition principle of the TAP transporter disclosed by combinatorial peptide libraries.

4. Regulation of Protein Degradation.

5. Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus.

6. Purification and Characterization of Two Functional Forms of Intracellular Protease PfpI from the Hyperthermophilic Archaeon Pyrococcus furiosus.

7. Intracellular peptides as natural regulators of cell signaling.

8. Computational prediction of cleavage using proteasomal in vitro digestion and MHC I ligand data.

9. Optimal length transportation hypothesis to model proteasome product size distribution.

10. Characterization of the Ovarian Tumor Peptidome.