[EFFect of alpha-ketoglutarate and acetylcholine synergism on energy metabolism in mitochondria].

The influence of Na alpha-ketoglutarate intraperioneally injected in dose of 20 mg per 100 g of body weight on substrate oxidation in the rat liver and heart mitochondria, cholinesterase activity in the blood, liver and pancreas and acetylcholine level in the rat liver and pancreas has been studied. Alpha-ketoglutarate is found to have a pronounced reciprocal effect on intramitochondrial alpha-ketoglutarate and succinate oxidation (acceleration and inhibition, respectively). The effect of Na alpha-ketoglutarate is specific for the two substrates as it is absent when another substrate of the tricarboxylic acid cycle is oxidized. This selective influence on substrate oxidation is realized by means of activation of aminotransferase reaction, which lead to alpha-ketoglutarate production by transamination. This effect is almost the same as in case of acetylcholine injection and exceeds it for the value of some energy parameters. It is supposed that influence of Na alpha-ketoglutarate on energy metabolism is realized by means of cholinesterase inhibition and of acetylcholine level increase. The stimulation effect on oxidative phosphorylation is mostly expressed in case of joint injection of Na alpha-ketoglutarate and acetylcholine. It is supposed that Na alpha-ketoglutarate is a synergist to acetylcholine.