Literature DB >> 8043650

Comparison of solution structure of free and complexed lac operator by molecular modelling with NMR constraints.

E Gincel1, G Lancelot, J C Maurizot, N T Thuong, F Vovelle.   

Abstract

The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator adopt both a right-handed B helical conformation, but a more detailed analysis of the conformational parameters using the Curves program shows striking differences in the groove geometries, the rises, the twists and the total bending.

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Year:  1994        PMID： 8043650     DOI： 10.1016/0300-9084(94)90006-x

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  4 in total

1.  The experimental folding landscape of monomeric lactose repressor, a large two-domain protein, involves two kinetic intermediates.

Authors:  Corey J Wilson; Payel Das; Cecilia Clementi; Kathleen S Matthews; Pernilla Wittung-Stafshede
Journal:  Proc Natl Acad Sci U S A       Date:  2005-10-03       Impact factor: 11.205

2.  Selectively 13C-enriched DNA: evidence from 13C1' relaxation rate measurements of an internal dynamics sequence effect in the lac operator.

Authors:  F Paquet; F Gaudin; G Lancelot
Journal:  J Biomol NMR       Date:  1996-10       Impact factor: 2.835

3.  Reorganization of terminator DNA upon binding replication terminator protein: implications for the functional replication fork arrest complex.

Authors:  A V Kralicek; P K Wilson; G B Ralston; R G Wake; G F King
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

4.  Solution structure of oligonucleotides covalently linked to a psoralen derivative.

Authors:  O Bornet; C Prévost; F Vovelle; M Chassignol; N T Thuong; G Lancelot
Journal:  Nucleic Acids Res       Date:  1995-03-11       Impact factor: 16.971
  4 in total

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