| Literature DB >> 804322 |
A P Eker, A M Fichtinger-Schepman.
Abstract
A procedure is described for the isolation of a DNA photoreactivating enzyme from Streptomyces griseus. Application of chromatography on spherosil, ultraviolet-irradiated DNA-cellulose, DEAE-cellulose and single stranded-DNA-agarose resulted in a 22 000-fold purification with 46 percent recovery on the initial activity. According to polyacrylamide gel electrophoresis the final preparation was virtually homogeneous. The absorption spectrum of the enzyme exhibited a marked absorption band in the 400-460 nm region in addition to protein absorption.Entities:
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Year: 1975 PMID： 804322 DOI： 10.1016/0005-2787(75)90136-7
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002