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Literature DB >> 8042903

Characterization of the Rhizobium leguminosarum biovar phaseoli nifA gene, a positive regulator of nif gene expression.

J Michiels¹, I D'hooghe, C Verreth, H Pelemans, J Vanderleyden.

Abstract

We report the isolation, mutational analysis and the nucleotide sequence of the Rhizobium leguminosarum bv. phaseoli nifA gene. Comparison of the deduced amino acid sequence with other NifA sequences indicated the presence of the conserved central activator and the C-terminal DNA-binding domains. Nodules elicited by a R. leguminosarum bv. phaseoli nifA mutant were symbiotically ineffective. The expression of a nifA-gusA fusion was shown to be independent on the oxygen status of the cell. We cloned the three nifH copies of R. leguminosarum bv. phaseoli and determined the nucleotide sequence of their promoter regions. The expression of nifH-gusA fusions is induced under microaerobic conditions and is dependent on the presence of NifA.

Entities: Chemical Species

Mesh：

Substances：
DNA, Bacterial

Year: 1994 PMID： 8042903 DOI： 10.1007/bf00288950

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

20 in total

1. Involvement of fixLJ in the regulation of nitrogen fixation in Azorhizobium caulinodans.

Authors: P A Kaminski; C Elmerich
Journal: Mol Microbiol Date: 1991-03 Impact factor: 3.501

2. Essential and non-essential domains in the Bradyrhizobium japonicum NifA protein: identification of indispensable cysteine residues potentially involved in redox reactivity and/or metal binding.

Authors: H M Fischer; T Bruderer; H Hennecke
Journal: Nucleic Acids Res Date: 1988-03-25 Impact factor: 16.971

3. Overlapping transcription of the nifA regulatory gene in Rhizobium meliloti.

Authors: C H Kim; D R Helinski; G Ditta
Journal: Gene Date: 1986 Impact factor: 3.688

4. Cloning and sequence of the Rhizobium leguminosarum biovar phaseoli fixA gene.

Authors: J Michiels; J Vanderleyden
Journal: Biochim Biophys Acta Date: 1993-09-13

5. Cascade regulation of nif gene expression in Rhizobium meliloti.

Authors: M David; M L Daveran; J Batut; A Dedieu; O Domergue; J Ghai; C Hertig; P Boistard; D Kahn
Journal: Cell Date: 1988-08-26 Impact factor: 41.582

6. Identification of two classes of Rhizobium phaseoli genes required for melanin synthesis, one of which is required for nitrogen fixation and activates the transcription of the other.

Authors: D Borthakur; J W Lamb; A W Johnston
Journal: Mol Gen Genet Date: 1987-04

7. A defined amino acid exchange close to the putative nucleotide binding site is responsible for an oxygen-tolerant variant of the Rhizobium meliloti NifA protein.

Authors: R Krey; A Pühler; W Klipp
Journal: Mol Gen Genet Date: 1992-09

8. Identification of a nifA-like regulatory gene of Azospirillum brasilense Sp7 expressed under conditions of nitrogen fixation and in the presence of air and ammonia.

Authors: Y Y Liang; P A Kaminski; C Elmerich
Journal: Mol Microbiol Date: 1991-11 Impact factor: 3.501

9. Broad host range and promoter selection vectors for bacteria that interact with plants.

Authors: G Van den Eede; R Deblaere; K Goethals; M Van Montagu; M Holsters
Journal: Mol Plant Microbe Interact Date: 1992 May-Jun Impact factor: 4.171

10. fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti.

Authors: J Batut; M L Daveran-Mingot; M David; J Jacobs; A M Garnerone; D Kahn
Journal: EMBO J Date: 1989-04 Impact factor: 11.598

17 in total

1. Regulatory role of Rhizobium etli CNPAF512 fnrN during symbiosis.

Authors: Martine Moris; Bruno Dombrecht; Chuanwu Xi; Jos Vanderleyden; Jan Michiels
Journal: Appl Environ Microbiol Date: 2004-03 Impact factor: 4.792

2. Regulatory proteins and cis-acting elements involved in the transcriptional control of Rhizobium etli reiterated nifH genes.

Authors: B Valderrama; A Dávalos; L Girard; E Morett; J Mora
Journal: J Bacteriol Date: 1996-06 Impact factor: 3.490

3. Mutagenesis and functional characterization of the glnB, glnA, and nifA genes from the photosynthetic bacterium Rhodospirillum rubrum.

Authors: Y Zhang; E L Pohlmann; P W Ludden; G P Roberts
Journal: J Bacteriol Date: 2000-02 Impact factor: 3.490

Characterization of the Rhizobium leguminosarum biovar phaseoli nifA gene, a positive regulator of nif gene expression.

1. Involvement of fixLJ in the regulation of nitrogen fixation in Azorhizobium caulinodans.

2. Essential and non-essential domains in the Bradyrhizobium japonicum NifA protein: identification of indispensable cysteine residues potentially involved in redox reactivity and/or metal binding.

3. Overlapping transcription of the nifA regulatory gene in Rhizobium meliloti.

4. Cloning and sequence of the Rhizobium leguminosarum biovar phaseoli fixA gene.

5. Cascade regulation of nif gene expression in Rhizobium meliloti.

6. Identification of two classes of Rhizobium phaseoli genes required for melanin synthesis, one of which is required for nitrogen fixation and activates the transcription of the other.

7. A defined amino acid exchange close to the putative nucleotide binding site is responsible for an oxygen-tolerant variant of the Rhizobium meliloti NifA protein.

8. Identification of a nifA-like regulatory gene of Azospirillum brasilense Sp7 expressed under conditions of nitrogen fixation and in the presence of air and ammonia.

9. Broad host range and promoter selection vectors for bacteria that interact with plants.

10. fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti.

1. Regulatory role of Rhizobium etli CNPAF512 fnrN during symbiosis.

2. Regulatory proteins and cis-acting elements involved in the transcriptional control of Rhizobium etli reiterated nifH genes.

3. Mutagenesis and functional characterization of the glnB, glnA, and nifA genes from the photosynthetic bacterium Rhodospirillum rubrum.

4. (Methyl)ammonium transport in the nitrogen-fixing bacterium Azospirillum brasilense.

5. Use of Dual Marker Transposons to Identify New Symbiosis Genes in Rhizobium.

Review 6. Genetic regulation of nitrogen fixation in rhizobia.

7. Expression of the fixR-nifA operon in Bradyrhizobium japonicum depends on a new response regulator, RegR.

8. Molecular basis of the establishment and functioning of a N2-fixing root nodule.

9. Structural and functional analysis of the fixLJ genes of Rhizobium leguminosarum biovar phaseoli CNPAF512.

10. Differential regulation of Rhizobium etli rpoN2 gene expression during symbiosis and free-living growth.