Literature DB >> 8041629

Sequencing of oligonucleotide phosphorothioates based on solid-supported desulfurization.

T K Wyrzykiewicz1, D L Cole.   

Abstract

We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligonucleotide phosphorothioates, anchored via an ester linkage to a standard solid support (LCAA/CPG), is combined with Maxam-Gilbert solid-support sequencing. The overall procedure allows rapid simultaneous sequence analysis of several oligonucleotide analogs.

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Year:  1994        PMID： 8041629      PMCID： PMC308225          DOI： 10.1093/nar/22.13.2667

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

Review 1.  Applications of mass spectrometry to DNA sequencing.

Authors:  K B Jacobson; H F Arlinghaus; M V Buchanan; C H Chen; G L Glish; R L Hettich; S A McLuckey
Journal:  Genet Anal Tech Appl       Date:  1991-12

2.  A reliable mapping method for sequence determination of oligodeoxyribonucleotides by mobility shift analysis.

Authors:  C P Tu; E Jay; C P Bahl; R Wu
Journal:  Anal Biochem       Date:  1976-07       Impact factor: 3.365

3.  Imaging plate illuminates many fields.

Authors:  Y Amemiya; J Miyahara
Journal:  Nature       Date:  1988-11-03       Impact factor: 49.962

4.  Solid supported hydrolysis of apurinic sites in synthetic oligonucleotides for rapid and efficient purification on reverse-phase cartridges.

Authors:  T Horn; M S Urdea
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

5.  Solid-phase methods for sequencing of nucleic acids I. Simultaneous sequencing of different oligodeoxyribonucleotides using a new, mechanically stable anion-exchange paper.

Authors:  A Rosenthal; S Schwertner; V Hahn; H D Hunger
Journal:  Nucleic Acids Res       Date:  1985-02-25       Impact factor: 16.971

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site.

Authors:  B A Connolly; B V Potter; F Eckstein; A Pingoud; L Grotjahn
Journal:  Biochemistry       Date:  1984-07-17       Impact factor: 3.162

8.  Regulatory concerns for the chemistry, manufacturing, and controls of oligonucleotide therapeutics for use in clinical studies.

Authors:  R V Kambhampati; Y Y Chiu; C W Chen; J J Blumenstein
Journal:  Antisense Res Dev       Date:  1993

9.  Solid phase synthesis of oligodeoxyribonucleoside phosphorodithioates from thiophosphoramidites.

Authors:  K Bjergårde; O Dahl
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

10.  Stereochemistry of internucleotide bond formation by polynucleotide phosphorylase from Micrococcus luteus.

Authors:  P M Burgers; F Eckstein
Journal:  Biochemistry       Date:  1979-02-06       Impact factor: 3.162
  10 in total
  1 in total

1.  Method for phosphorothioate antisense DNA sequencing by capillary electrophoresis with UV detection.

Authors:  D Froim; C E Hopkins; A Belenky; A S Cohen
Journal:  Nucleic Acids Res       Date:  1997-11-01       Impact factor: 16.971
  1 in total

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