Brain stem chemosensitivity: its implication in central respiratory regulation.

Central brain stem chemosensitivity plays an essential role in acid-base regulation. The site of the chemosensitive neuronal elements has not yet been clearly established. To address this question, we used an in vitro adult guinea pig isolated brain stem preparation, maintained in survival conditions by intrabasilar and bath perfusions of Krebs solution saturated with 95% O2 and 5% CO2. Hypercapnic stimulation, produced by modifying the CO2 concentration of the medium perfusing brain stem structures via the basilar perfusion system, increased the respiratory burst frequency recorded from the hypoglossal nerve. Hypercapnia evoked by ventral surface superfusion increased the respiratory burst amplitude. These data suggest that chemosensitive neuronal elements could be located at the ventral surface as well as in the deeper brainstem structures. However, their characteristics may be different.