Literature DB >> 8036170

The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

A Fomenkov1, J P Xiao, D Dila, E Raleigh, S Y Xu.   

Abstract

A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E. coli cells are not fully protected by the cognate methylase, the restriction enzyme damages the DNA in vivo and induces the SOS response. The SOS-induced cells form blue colonies on indicator plates containing X-gal. Using this method the genes coding for the thermostable restriction enzymes Taql (5'TCGA3') and Tth111l (5'GACNNNGTC3') have been successfully cloned in E. coli. The new strain will be useful to clone other genes involved in DNA metabolism.

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Year:  1994        PMID： 8036170      PMCID： PMC523701          DOI： 10.1093/nar/22.12.2399

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  27 in total

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Authors:  G G Wilson
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

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Journal:  Proteins       Date:  1990

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Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

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Authors:  G G Wilson
Journal:  Gene       Date:  1988-12-25       Impact factor: 3.688

5.  Tn10 transposition promotes RecA-dependent induction of a lambda prophage.

Authors:  D Roberts; N Kleckner
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

6.  Characterization and expression of the Escherichia coli Mrr restriction system.

Authors:  P A Waite-Rees; C J Keating; L S Moran; B E Slatko; L J Hornstra; J S Benner
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

7.  A new method for the rapid identification of genes encoding restriction and modification enzymes.

Authors:  A Piekarowicz; R Yuan; D C Stein
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

8.  Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12.

Authors:  A Piekarowicz; R Yuan; D C Stein
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

9.  Cofactor requirements of BamHI mutant endonuclease E77K and its suppressor mutants.

Authors:  S Y Xu; I Schildkraut
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

10.  Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.

Authors:  J Heitman; P Model
Journal:  EMBO J       Date:  1990-10       Impact factor: 11.598
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  17 in total

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Authors:  James C Samuelson; Zhenyu Zhu; Shuang-yong Xu
Journal:  Nucleic Acids Res       Date:  2004-07-09       Impact factor: 16.971

2.  Cloning of the BssHII restriction-modification system in Escherichia coli : BssHII methyltransferase contains circularly permuted cytosine-5 methyltransferase motifs.

Authors:  S Xu; J Xiao; J Posfai; R Maunus; J Benner
Journal:  Nucleic Acids Res       Date:  1997-10-15       Impact factor: 16.971

3.  Rational design of a chimeric endonuclease targeted to NotI recognition site.

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4.  Expression and purification of BmrI restriction endonuclease and its N-terminal cleavage domain variants.

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5.  Functional analysis of MmeI from methanol utilizer Methylophilus methylotrophus, a subtype IIC restriction-modification enzyme related to type I enzymes.

Authors:  Joanna Nakonieczna; Tadeusz Kaczorowski; Agnieszka Obarska-Kosinska; Janusz M Bujnicki
Journal:  Appl Environ Microbiol       Date:  2008-11-07       Impact factor: 4.792

6.  In vivo DNA protection by relaxed-specificity SinI DNA methyltransferase variants.

Authors:  Edit Tímár; Pál Venetianer; Antal Kiss
Journal:  J Bacteriol       Date:  2008-10-10       Impact factor: 3.490

7.  Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems.

Authors:  H Ruan; K D Lunnen; M E Scott; L S Moran; B E Slatko; J J Pelletier; E J Hess; J Benner; G G Wilson; S Y Xu
Journal:  Mol Gen Genet       Date:  1996-10-28

8.  Yeast and human genes that affect the Escherichia coli SOS response.

Authors:  E L Perkins; J F Sterling; V I Hashem; M A Resnick
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-02       Impact factor: 11.205

9.  Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification.

Authors:  Siu-hong Chan; Zhenyu Zhu; James L Van Etten; Shuang-yong Xu
Journal:  Nucleic Acids Res       Date:  2004-11-29       Impact factor: 16.971

10.  Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease.

Authors:  Brian P Anton; Elisabeth A Raleigh
Journal:  J Bacteriol       Date:  2004-09       Impact factor: 3.490
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