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Literature DB >> 803565

Bacteriophage transformation of PBS2 in Bacillus subtilis.

Abstract

Transformation of temperature-sensitive mutants of bacteriophage PBS2 for Bacillus subtilis was demonstrated. The number of transformants was linearly related to the concentration of DNA within a range of 0.01 to 1 mug/ml. No transformants were obtained when the DNA was pretreated with DNase. PBS2 DNA sheared to approximately 1% of the total chromosome length was centrifuged in Cs2SO4-Hg gradients to fractionate the DNA according to the base composition. Transformation experiments carried out with the fractionated DNA indicated the possibility of determining the base composition of different regions of the phage chromosome.

Entities: Chemical Species

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Year: 1975 PMID： 803565 PMCID： PMC354411 DOI： 10.1128/JVI.15.1.16-21.1975

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

16 in total

1. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82.

Authors: D M GREEN
Journal: J Mol Biol Date: 1964-12 Impact factor: 5.469

2. Replacement of thymidylic acid by deoxyuridylic acid in the deoxyribonucleic acid of a transducing phage for Bacillus subtilis.

Authors: I TAKAHASHI; J MARMUR
Journal: Nature Date: 1963-02-23 Impact factor: 49.962

1. Influence of bacteriophage PBS1 and phi W-14 deoxyribonucleic acids on homologous deoxyribonucleic acid uptake and transformation in competent Bacillus subtilis.

Authors: P López; M Espinosa; M Piechowska; D Shugar
Journal: J Bacteriol Date: 1980-07 Impact factor: 3.490

1 in total

Bacteriophage transformation of PBS2 in Bacillus subtilis.

1. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82.

2. Replacement of thymidylic acid by deoxyuridylic acid in the deoxyribonucleic acid of a transducing phage for Bacillus subtilis.

3. Transducing phages for Bacillus subtilis.

4. ON THE SIZE OF GENETIC DETERMINANTS IN PNEUMOCOCCUS AND THE NATURE OF THE VARIABLES INVOLVED IN TRANSFORMATION.

5. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.

6. Gene Linkage in DNA Transfer: A Cluster of Genes Concerned with Aromatic Biosynthesis in Bacillus Subtilis.

7. Transducing particles of PBS 1.

8. Segmental distribution of nucleotides in the DNA of bacteriophage lambda.

9. LOCALIZATION OF SPORE MARKERS ON THE CHROMOSOME OF BACILLUS SUBTILIS.

10. TRANSDUCTION OF SPOROGENESIS IN BACILLUS SUBTILIS.

1. Influence of bacteriophage PBS1 and phi W-14 deoxyribonucleic acids on homologous deoxyribonucleic acid uptake and transformation in competent Bacillus subtilis.