| Literature DB >> 8034033 |
J A Killian1, T P Trouard, D V Greathouse, V Chupin, G Lindblom.
Abstract
A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.Entities:
Mesh：
Substances：
Year: 1994 PMID： 8034033 DOI： 10.1016/0014-5793(94)00594-x
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124