Literature DB >> 8030787

Determination of binding constants of ligands to proteins by affinity capillary electrophoresis: compensation for electroosmotic flow.

F A Gomez1, L Z Avila, Y H Chu, G M Whitesides.   

Abstract

This paper describes the estimation of binding constants (Kb) between carbonic anhydrase B (CAB, EC 4.2.1.1, from bovine erythrocytes) and charged benzenesulfonamides by affinity capillary electrophoresis (ACE) under conditions in which the migration time is affected by changes in electroosmotic flow and by nonspecific interactions accompanying changes in the concentration of ligand. Comparisons of values of migration times of the protein of interest, and of "noninteracting" marker proteins, with those of a neutral internal standard provide the basis for corrections for variable electroosmotic flow; these corrections make possible the estimation of Kb and its uncertainty even in the presence of substantial variations in electroosmotic flow.

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Year:  1994        PMID： 8030787     DOI： 10.1021/ac00083a003

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  9 in total

Review 1.  Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding.

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2.  Detection of noncovalent FKBP-FK506 and FKBP-Rapamycin complexes by capillary electrophoresis-mass spectrometry and capillary electrophoresis-tandem mass spectrometry.

Authors:  Y L Hsieh; J Cai; Y T Li; J D Henion; B Ganem
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3.  Optimization of a microfluidic electrophoretic immunoassay using a Peltier cooler.

Authors:  Nikita Mukhitov; Lian Yi; Adrian M Schrell; Michael G Roper
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4.  Design of peptide affinity ligands for S-protein: a comparison of combinatorial and de novo design strategies.

Authors:  Divya Chandra; Christopher J Morrison; James Woo; Steven Cramer; Pankaj Karande
Journal:  Mol Divers       Date:  2013-03-27       Impact factor: 2.943

5.  Determination of the effective charge of a protein in solution by capillary electrophoresis.

Authors:  J Gao; F A Gomez; R Härter; G M Whitesides
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-06       Impact factor: 11.205

6.  Glycosylation provides both stimulatory and inhibitory effects on cell surface and soluble CD44 binding to hyaluronan.

Authors:  T P Skelton; C Zeng; A Nocks; I Stamenkovic
Journal:  J Cell Biol       Date:  1998-01-26       Impact factor: 10.539

7.  Molecular interactions of a semisynthetic glycopeptide antibiotic with D-alanyl-D-alanine and D-alanyl-D-lactate residues.

Authors:  N E Allen; D L LeTourneau; J N Hobbs
Journal:  Antimicrob Agents Chemother       Date:  1997-01       Impact factor: 5.191

8.  Hexapeptide derivatives of glycopeptide antibiotics: tools for mechanism of action studies.

Authors:  Norris E Allen; Deborah L LeTourneau; Joe N Hobbs; Richard C Thompson
Journal:  Antimicrob Agents Chemother       Date:  2002-08       Impact factor: 5.191

9.  Combined use of chiral ionic liquid and CD for MEKC: Part II. Determination of binding constants.

Authors:  Bin Wang; Jun He; Victoria Bianchi; Shahab A Shamsi
Journal:  Electrophoresis       Date:  2009-08       Impact factor: 3.535
  9 in total

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