Literature DB >> 8030200

Detection of the readthrough protein of barley yellow dwarf virus.

S L Cheng1, L L Domier, C J D'Arcy.   

Abstract

The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) raised against BYDV-PAV-IL could specifically detect the presence of the 72-kDa readthrough protein in extracts from the BYDV-infected leaf tissue. The results suggest that ORF 5 (50-kDa protein) is translated by readthrough of ORF 3 (22-kDa coat protein) to produce the 72-kDa protein. The readthrough protein is thought to be a structural protein on the external surface of BYDV.

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Year:  1994        PMID： 8030200     DOI： 10.1006/viro.1994.1427

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  6 in total

1.  Local and distant sequences are required for efficient readthrough of the barley yellow dwarf virus PAV coat protein gene stop codon.

Authors:  C M Brown; S P Dinesh-Kumar; W A Miller
Journal:  J Virol       Date:  1996-09       Impact factor: 5.103

2.  Cross-linking measurements of the Potato leafroll virus reveal protein interaction topologies required for virion stability, aphid transmission, and virus-plant interactions.

Authors:  Juan D Chavez; Michelle Cilia; Chad R Weisbrod; Ho-Jong Ju; Jimmy K Eng; Stewart M Gray; James E Bruce
Journal:  J Proteome Res       Date:  2012-03-30       Impact factor: 4.466

3.  Effects of point mutations in the major capsid protein of beet western yellows virus on capsid formation, virus accumulation, and aphid transmission.

Authors:  V Brault; M Bergdoll; J Mutterer; V Prasad; S Pfeffer; M Erdinger; K E Richards; V Ziegler-Graff
Journal:  J Virol       Date:  2003-03       Impact factor: 5.103

Review 4.  Principles of molecular organization, expression, and evolution of closteroviruses: over the barriers.

Authors:  A A Agranovsky
Journal:  Adv Virus Res       Date:  1996       Impact factor: 9.937

5.  Both structural and non-structural forms of the readthrough protein of cucurbit aphid-borne yellows virus are essential for efficient systemic infection of plants.

Authors:  Sylvaine Boissinot; Monique Erdinger; Baptiste Monsion; Véronique Ziegler-Graff; Véronique Brault
Journal:  PLoS One       Date:  2014-04-01       Impact factor: 3.240

6.  Evidence for lysine acetylation in the coat protein of a polerovirus.

Authors:  Michelle Cilia; Richard Johnson; Michelle Sweeney; Stacy L DeBlasio; James E Bruce; Michael J MacCoss; Stewart M Gray
Journal:  J Gen Virol       Date:  2014-06-17       Impact factor: 3.891
  6 in total

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