Literature DB >> 8025675

A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae.

C S Shankar1, S Umesh-Kumar.   

Abstract

A cell-wall-surface protein purified from the cells of Saccharomyces cerevisiae NCYC 227 was found to be involved in the non-sexual flocculation of this yeast. This 13 kDa protein was found to bind specifically to mannose. The protein bound to mannans isolated from yeast as well as in situ to intact cells, but only in the presence of calcium ions. The protein, a mannoprotein, formed aggregates as revealed in SDS-PAGE. Urea and higher temperatures prevented protein aggregation, suggesting that the flocculation of S. cerevisiae is primarily due to hydrogen bonding between mannan and protein.

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Year:  1994        PMID: 8025675     DOI: 10.1099/13500872-140-5-1097

Source DB:  PubMed          Journal:  Microbiology        ISSN: 1350-0872            Impact factor:   2.777


  8 in total

1.  Mitochondrial function in cell wall glycoprotein synthesis in Saccharomyces cerevisiae NCYC 625 (Wild type) and [rho(0)] mutants.

Authors:  A R Iung; J Coulon; F Kiss; J N Ekome; J Vallner; R Bonaly
Journal:  Appl Environ Microbiol       Date:  1999-12       Impact factor: 4.792

2.  The Saccharomyces cerevisiae MADS-box transcription factor Rlm1 is a target for the Mpk1 mitogen-activated protein kinase pathway.

Authors:  E Dodou; R Treisman
Journal:  Mol Cell Biol       Date:  1997-04       Impact factor: 4.272

3.  Expression and characterization of the flocculin Flo11/Muc1, a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion.

Authors:  Lois M Douglas; Li Li; Yang Yang; A M Dranginis
Journal:  Eukaryot Cell       Date:  2007-10-05

4.  Region of FLO1 proteins responsible for sugar recognition.

Authors:  O Kobayashi; N Hayashi; R Kuroki; H Sone
Journal:  J Bacteriol       Date:  1998-12       Impact factor: 3.490

5.  Mechanisms of yeast flocculation: comparison of top- and bottom-fermenting strains.

Authors:  P B Dengis; L R Nélissen; P G Rouxhet
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

6.  Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p.

Authors:  M Bony; D Thines-Sempoux; P Barre; B Blondin
Journal:  J Bacteriol       Date:  1997-08       Impact factor: 3.490

7.  Yeast cell adhesion molecules have functional amyloid-forming sequences.

Authors:  Caleen B Ramsook; Cho Tan; Melissa C Garcia; Raymond Fung; Gregory Soybelman; Ryan Henry; Anna Litewka; Shanique O'Meally; Henry N Otoo; Roy A Khalaf; Anne M Dranginis; Nand K Gaur; Stephen A Klotz; Jason M Rauceo; Chong K Jue; Peter N Lipke
Journal:  Eukaryot Cell       Date:  2009-12-28

8.  LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast.

Authors:  Yoon-Dong Park; Soo Jeong Kwon; Kyung Sook Bae; Hee-Moon Park
Journal:  Mycobiology       Date:  2018-09-30       Impact factor: 1.858

  8 in total

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