Literature DB >> 802511

Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.

D Eilat, E Ziv, Y Lapidot.   

Abstract

The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.

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Year:  1975        PMID： 802511      PMCID： PMC343591          DOI： 10.1093/nar/2.12.2237

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid.

Authors:  S Friedman
Journal:  Biochemistry       Date:  1972-08-29       Impact factor: 3.162

2.  Patterns of base modification in tRNA.

Authors:  S Friedman
Journal:  Nat New Biol       Date:  1973-07-04

3.  Isolation of isoaccepting tRNAs.

Authors:  L Sokolff; H P Rappaport
Journal:  Arch Biochem Biophys       Date:  1972-12       Impact factor: 4.013

Review 4.  The chemical synthesis and the biochemical properties of peptidyl-tRNA.

Authors:  Y Lapidot; N de Groot
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1972

5.  Peptidyl-tRNA. XII. Behaviour of peptidyl-tRNA-Phe from Escherichia coli on methylated albumin silicic acid column.

Authors:  E Ziv; N De Groot; Y Lapidot
Journal:  Biochim Biophys Acta       Date:  1971-01-01

6.  The synthesis of oligopeptidyl-tRNA.

Authors:  Y Lapidot; S Rappoport
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

7.  Fractionation of Escherichia coli transfer RNA on benzoylated DEAE-cellulose.

Authors:  K L Roy; D Söll
Journal:  Biochim Biophys Acta       Date:  1968-07-23

8.  Peptidyl transfer RNA. 8. The chemical synthesis of glycine and alanine containing oligopeptidyl transfer RNA.

Authors:  Y Lapidot; N De Groot; S Rappoport; D Elat
Journal:  Biochim Biophys Acta       Date:  1969-10-22

9.  Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA.

Authors:  Y Lapidot; D Elat; S Rappoport; N de Groot
Journal:  Biochem Biophys Res Commun       Date:  1970-02-20       Impact factor: 3.575

10.  The binding of Phe-tRNA(Phe) and gly(2)Phe-tRNA(Phe) to reticulocyte ribosomal peptidyl sites by a mechanism not involving translocation.

Authors:  H Rahamimoff; E Baksht; Y Lapidot; N de Groot
Journal:  FEBS Lett       Date:  1972-05-01       Impact factor: 4.124
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