Literature DB >> 802472

A general method for enumerating single cells secreting antigen: albumin-secreting hepatocytes detected as plaque-forming cells.

G A Molinaro, E Maron, W C Eby, S Dray.   

Abstract

Sheep erythrocytes, artificially coated with purified antibody specific for mouse serum albumin (anti-MSA Ab-E) were agglutinated and lysed by mouse serum albumin. Mouse liver cells, plated with anti-MSA Ab-E formed hemolytic plaque in a modified plaque-forming cell (PFC) assay. The same cells tested with anti-MSA Ab-E did not rosette in modified immunocyto-adhesion assay. Thus, liver cells secreted molecules (albumin) which were not detectable on the cell membrane. This PFC assay with Ab-E is probably a general method for enumerating antigen-secreting cell.

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Year:  1975        PMID： 802472     DOI： 10.1002/eji.1830051108

Source DB:  PubMed          Journal:  Eur J Immunol        ISSN： 0014-2980            Impact factor:   5.532


  7 in total

1.  IgM RNA switch from membrane to secretory form is prevented by adding antireceptor antibody to bacterial lipopolysaccharide-stimulated murine primary B-cell cultures.

Authors:  U Chen-Bettecken; E Wecker; A Schimpl
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

2.  Isolation of cells secreting specific polypeptides.

Authors:  M A Feldman; J Y Chou
Journal:  In Vitro       Date:  1983-03

3.  Use of in situ hybridization to identify collagen and albumin mRNAs in isolated mouse hepatocytes.

Authors:  M A Saber; M A Zern; D A Shafritz
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

4.  Opposite effects of BCG on spleen and lymph node cells: lymphocyte proliferation and immunoglobulin synthesis.

Authors:  R Turcotte; L Lafleur; M Labrèche
Journal:  Infect Immun       Date:  1978-09       Impact factor: 3.441

5.  Presence of normal human cell surface antigens in plasma of athymic mice bearing a human colon carcinoma and in normal human plasma.

Authors:  Y Markson; D W Weiss; O Weiss; F Doljanski
Journal:  Cancer Immunol Immunother       Date:  1985       Impact factor: 6.968

6.  Stimulation of human T lymphocytes by PHA-activated autologous T lymphocytes: analysis of the role of Ia-like antigens with monoclonal antibodies.

Authors:  C Russo; F Indiveri; V Quaranta; G A Molinaro; M A Pellegrino; S Ferrone
Journal:  Immunogenetics       Date:  1981       Impact factor: 2.846

7.  Changes in collagen and albumin mRNA in liver tissue of mice infected with Schistosoma mansoni as determined by in situ hybridization.

Authors:  M A Saber; D A Shafritz; M A Zern
Journal:  J Cell Biol       Date:  1983-10       Impact factor: 10.539
  7 in total

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