Literature DB >> 8024599

Peroxisomal acyl-coenzyme A oxidase is a rate-limiting enzyme in a very-long-chain fatty acid beta-oxidation system.

T Aoyama1, M Souri, T Kamijo, S Ushikubo, T Hashimoto.   

Abstract

The contents of peroxisomal fatty acid beta-oxidation enzymes in three rat hepatoma cell lines, i.e., H4IIEC3 (H4), N1S1, and McA-RH7777 (H7), were measured by immunoblot analysis, and a significant difference in acyl-coenzyme A oxidase (AOX) content became evident. These cell lines were respectively infected with a recombinant virus to express significant amounts of AOX protein. The expressed AOX mainly localized in organelle, supposing peroxisomes, and was catalytically active. The cDNA-expression in H4, N1S1, and H7 cells enhanced 2.6-, 2.2-, and 1.0-fold beta-oxidation activity of lignoceric acid, respectively. The enhancement in H4 and N1S1 cells suggests that AOX is a rate-limiting enzyme in the very-long-chain fatty acid beta-oxidation system, in these cell lines.

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Year:  1994        PMID： 8024599     DOI： 10.1006/bbrc.1994.1879

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Molecular characterization of mitochondrial trifunctional protein deficiency: formation of the enzyme complex is important for stabilization of both alpha- and beta-subunits.

Authors:  S Ushikubo; T Aoyama; T Kamijo; R J Wanders; P Rinaldo; J Vockley; T Hashimoto
Journal:  Am J Hum Genet       Date:  1996-05       Impact factor: 11.025

2.  Identification, separation, and characterization of acyl-coenzyme A dehydrogenases involved in mitochondrial beta-oxidation in higher plants

Authors: 
Journal:  Plant Physiol       Date:  1999-04       Impact factor: 8.340

3.  Arabidopsis lipins, PDAT1 acyltransferase, and SDP1 triacylglycerol lipase synergistically direct fatty acids toward β-oxidation, thereby maintaining membrane lipid homeostasis.

Authors:  Jilian Fan; Chengshi Yan; Rebecca Roston; John Shanklin; Changcheng Xu
Journal:  Plant Cell       Date:  2014-10-07       Impact factor: 11.277

4.  Higher-plant medium- and short-chain acyl-CoA oxidases: identification, purification and characterization of two novel enzymes of eukaryotic peroxisomal beta-oxidation.

Authors:  M A Hooks; K Bode; I Couée
Journal:  Biochem J       Date:  1996-12-01       Impact factor: 3.857

5.  T₃-induced liver AMP-activated protein kinase signaling: redox dependency and upregulation of downstream targets.

Authors:  Luis A Videla; Virginia Fernández; Pamela Cornejo; Romina Vargas; Paula Morales; Juan Ceballo; Alvaro Fischer; Nicolás Escudero; Oscar Escobar
Journal:  World J Gastroenterol       Date:  2014-12-14       Impact factor: 5.742

6.  Purification of human very-long-chain acyl-coenzyme A dehydrogenase and characterization of its deficiency in seven patients.

Authors:  T Aoyama; M Souri; S Ushikubo; T Kamijo; S Yamaguchi; R I Kelley; W J Rhead; K Uetake; K Tanaka; T Hashimoto
Journal:  J Clin Invest       Date:  1995-06       Impact factor: 14.808

7.  Life without white fat: a transgenic mouse.

Authors:  J Moitra; M M Mason; M Olive; D Krylov; O Gavrilova; B Marcus-Samuels; L Feigenbaum; E Lee; T Aoyama; M Eckhaus; M L Reitman; C Vinson
Journal:  Genes Dev       Date:  1998-10-15       Impact factor: 11.361

8.  Cloning of human very-long-chain acyl-coenzyme A dehydrogenase and molecular characterization of its deficiency in two patients.

Authors:  T Aoyama; M Souri; I Ueno; T Kamijo; S Yamaguchi; W J Rhead; K Tanaka; T Hashimoto
Journal:  Am J Hum Genet       Date:  1995-08       Impact factor: 11.025

9.  Lingonberry Improves Hepatic Lipid Metabolism by Targeting Notch1 Signaling.

Authors:  Susara Madduma Hewage; Kathy K W Au-Yeung; Suvira Prashar; Charith U B Wijerathne; Karmin O; Yaw L Siow
Journal:  Antioxidants (Basel)       Date:  2022-02-27
  9 in total

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