OBJECTIVE: To determine if oral fluid samples can be used to reliably assess protective blood levels of antibodies to measles, mumps, and rubella. DESIGN: A comparison of matched serum and oral fluid samples from asymptomatic subjects in enzyme-linked immunosorbent assays for measles, mumps, and rubella antibodies. A longitudinal study compared matched serum and oral fluid samples from 11 subjects after measles-mumps-rubella vaccination. SETTING: Five US clinical sites with samples tested at the reporting laboratory. PARTICIPANTS: A total of 157 asymptomatic subjects including 55 subjects younger than 18 years. MAIN OUTCOME MEASURES AND RESULTS: The presence of antibodies in oral fluid specimens correlated with that in serum with the following levels of sensitivity and specificity: measles, 97% and 100%, respectively; mumps, 94% and 94%, respectively; rubella, 98% and 98%, respectively. Longitudinal studies of subjects after vaccination showed similar seroconversion profiles in oral fluid and serum samples. CONCLUSION: Protective blood levels of antibodies to measles, mumps, and rubella can be assessed by means of an oral fluid sample with good reliability.
OBJECTIVE: To determine if oral fluid samples can be used to reliably assess protective blood levels of antibodies to measles, mumps, and rubella. DESIGN: A comparison of matched serum and oral fluid samples from asymptomatic subjects in enzyme-linked immunosorbent assays for measles, mumps, and rubella antibodies. A longitudinal study compared matched serum and oral fluid samples from 11 subjects after measles-mumps-rubella vaccination. SETTING: Five US clinical sites with samples tested at the reporting laboratory. PARTICIPANTS: A total of 157 asymptomatic subjects including 55 subjects younger than 18 years. MAIN OUTCOME MEASURES AND RESULTS: The presence of antibodies in oral fluid specimens correlated with that in serum with the following levels of sensitivity and specificity: measles, 97% and 100%, respectively; mumps, 94% and 94%, respectively; rubella, 98% and 98%, respectively. Longitudinal studies of subjects after vaccination showed similar seroconversion profiles in oral fluid and serum samples. CONCLUSION: Protective blood levels of antibodies to measles, mumps, and rubella can be assessed by means of an oral fluid sample with good reliability.
Authors: K Vainio; H H Samdal; G Anestad; E Wedege; D H Skutlaberg; K T Bransdal; R Mundal; I S Aaberge Journal: Eur J Clin Microbiol Infect Dis Date: 2008-02-22 Impact factor: 3.267
Authors: Grace O Gbotosho; Christian T Happi; Onikepe Folarin; Ochuko Keyamo; Akintunde Sowunmi; Ayoade M J Oduola Journal: Am J Trop Med Hyg Date: 2010-09 Impact factor: 2.345
Authors: Tobias O Apinjoh; Veronica N Ntasin; Phil Collins C Tataw; Vincent N Ntui; Dieudonne L Njimoh; Fidelis Cho-Ngwa; Eric A Achidi Journal: Infect Dis Poverty Date: 2021-05-22 Impact factor: 4.520