Literature DB >> 8021180

Two different dihydroorotate dehydrogenases in Lactococcus lactis.

P S Andersen1, P J Jansen, K Hammer.   

Abstract

The pyrimidine de novo biosynthesis pathway has been characterized for a number of organisms. The general pathway consists of six enzymatic steps. In the characterization of the pyrimidine pathway of Lactococcus lactis, two different pyrD genes encoding dihydroorotate dehydrogenase were isolated. The nucleotide sequences of the two genes, pyrDa and pyrDb, have been determined. One of the deduced amino acid sequences has a high degree of homology to the Saccharomyces cerevisiae dihydroorotate dehydrogenase, and the other resembles the dihydroorotate dehydrogenase from Bacillus subtilis. It is possible to distinguish between the two enzymes in crude extracts by using different electron acceptors. We constructed mutants containing a mutated form of either one or the other or both of the pyrD genes. Only the double mutant is pyrimidine auxotrophic.

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Year:  1994        PMID： 8021180      PMCID： PMC205595          DOI： 10.1128/jb.176.13.3975-3982.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  31 in total

1.  Dihydro-orotate oxidase of Escherichia coli K12: purification, properties, and relation to the cytoplasmic membrane.

Authors:  D Karibian; P Couchoud
Journal:  Biochim Biophys Acta       Date:  1974-10-17

2.  An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis.

Authors:  B Nilsson; M Uhlén; S Josephson; S Gatenbeck; L Philipson
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

3.  Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.

Authors:  D Simon; A Chopin
Journal:  Biochimie       Date:  1988-04       Impact factor: 4.079

4.  Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.

Authors:  C L Quinn; B T Stephenson; R L Switzer
Journal:  J Biol Chem       Date:  1991-05-15       Impact factor: 5.157

5.  Purification and properties of the bovine liver mitochondrial dihydroorotate dehydrogenase.

Authors:  V Hines; L D Keys; M Johnston
Journal:  J Biol Chem       Date:  1986-08-25       Impact factor: 5.157

6.  Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase.

Authors:  R M Llanos; C J Harris; A J Hillier; B E Davidson
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490

7.  Nucleotide sequence of the URA1 gene of Saccharomyces cerevisiae.

Authors:  A Roy
Journal:  Gene       Date:  1992-09-01       Impact factor: 3.688

8.  Biosynthetic dihydroorotate dehydrogenase from Lactobacillus bulgaricus: partial characterization of the enzyme.

Authors:  W H Taylor; C D Taylor; M L Taylor
Journal:  J Bacteriol       Date:  1974-07       Impact factor: 3.490

9.  Nucleotide sequence of the pyrD gene of Escherichia coli and characterization of the flavoprotein dihydroorotate dehydrogenase.

Authors:  J N Larsen; K F Jensen
Journal:  Eur J Biochem       Date:  1985-08-15

10.  Biosynthetic dihydroorotate dehydrogenase from Lactobacillus bulgaricus.

Authors:  M L Taylor; W H Taylor; D F Eames; C D Taylor
Journal:  J Bacteriol       Date:  1971-03       Impact factor: 3.490
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  18 in total

1.  The pyrimidine operon pyrRPB-carA from Lactococcus lactis.

Authors:  J Martinussen; J Schallert; B Andersen; K Hammer
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

2.  Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6.

Authors:  Joel A Lanie; Wai-Leung Ng; Krystyna M Kazmierczak; Tiffany M Andrzejewski; Tanja M Davidsen; Kyle J Wayne; Hervé Tettelin; John I Glass; Malcolm E Winkler
Journal:  J Bacteriol       Date:  2006-10-13       Impact factor: 3.490

3.  Identification of a novel gene of pyrimidine nucleotide biosynthesis, pyrDII, that is required for dihydroorotate dehydrogenase activity in Bacillus subtilis.

Authors:  A E Kahler; R L Switzer
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

4.  Anaerobic peroxisomes in Mastigamoeba balamuthi.

Authors:  Tien Le; Vojtěch Žárský; Eva Nývltová; Petr Rada; Karel Harant; Marie Vancová; Zdeněk Verner; Ivan Hrdý; Jan Tachezy
Journal:  Proc Natl Acad Sci U S A       Date:  2020-01-13       Impact factor: 11.205

5.  Cloning and characterization of upp, a gene encoding uracil phosphoribosyltransferase from Lactococcus lactis.

Authors:  J Martinussen; K Hammer
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

6.  Transcriptome analysis of Lactococcus lactis in coculture with Saccharomyces cerevisiae.

Authors:  Mathieu Maligoy; Myriam Mercade; Muriel Cocaign-Bousquet; Pascal Loubiere
Journal:  Appl Environ Microbiol       Date:  2007-11-09       Impact factor: 4.792

7.  The carB gene encoding the large subunit of carbamoylphosphate synthetase from Lactococcus lactis is transcribed monocistronically.

Authors:  J Martinussen; K Hammer
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

8.  Generation of auxotrophic mutants of Enterococcus faecalis.

Authors:  X Li; G M Weinstock; B E Murray
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

Review 9.  Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.

Authors:  Charles L Turnbough; Robert L Switzer
Journal:  Microbiol Mol Biol Rev       Date:  2008-06       Impact factor: 11.056

10.  The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function.

Authors:  P Rowland; O Björnberg; F S Nielsen; K F Jensen; S Larsen
Journal:  Protein Sci       Date:  1998-06       Impact factor: 6.725
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