Literature DB >> 8017946

Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

V Dartois1, J Y Coppée, C Colson, A Baulard.   

Abstract

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the delta lip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

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Year:  1994        PMID： 8017946      PMCID： PMC201535          DOI： 10.1128/aem.60.5.1670-1673.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

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