Literature DB >> 8016071

GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules.

D Bramhill1, C M Thompson.   

Abstract

The FtsZ protein is a GTPase that is essential for cell division in Escherichia coli. During cytokinesis, FtsZ localizes to a ring at the leading edge of septum synthesis. We report the GTP-dependent polymerization of purified FtsZ measured by sedimentation and light scattering. Electron microscopy of polymerized FtsZ revealed structures including tubules 14-20 nm in diameter with longitudinal arrays of protofilaments. FtsZ depolymerized upon removal of GTP and repolymerized after subsequent GTP addition. Mutant FtsZ84 protein polymerized inefficiently, suggesting that polymerization is important for the cellular role of FtsZ in division. The possibility that tubules of FtsZ protein form a cytoskeleton involved in septum synthesis is consistent with our data.

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Year:  1994        PMID： 8016071      PMCID： PMC44087          DOI： 10.1073/pnas.91.13.5813

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  45 in total

1.  FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coli.

Authors:  L M Guzman; J J Barondess; J Beckwith
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

2.  Inhibition of cell division initiation by an imbalance in the ratio of FtsA to FtsZ.

Authors:  S J Dewar; K J Begg; W D Donachie
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

3.  Escherichia coli mraR gene involved in cell growth and division.

Authors:  M Ueki; M Wachi; H K Jung; F Ishino; M Matsuhashi
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

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Journal:  Biochem Biophys Res Commun       Date:  1976-01-12       Impact factor: 3.575

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Authors:  R D Sloboda; W L Dentler; J L Rosenbaum
Journal:  Biochemistry       Date:  1976-10-05       Impact factor: 3.162

6.  The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

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Journal:  J Biol Chem       Date:  1971-08-10       Impact factor: 5.157

7.  Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules.

Authors:  F Gaskin; C R Cantor; M L Shelanski
Journal:  J Mol Biol       Date:  1974-11-15       Impact factor: 5.469

8.  Microtubule assembly in the absence of added nucleotides.

Authors:  M L Shelanski; F Gaskin; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

9.  Organization of genes in the ftsA-envA region of the Escherichia coli genetic map and identification of a new fts locus (ftsZ).

Authors:  J F Lutkenhaus; H Wolf-Watz; W D Donachie
Journal:  J Bacteriol       Date:  1980-05       Impact factor: 3.490

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Authors:  L Amos; A Klug
Journal:  J Cell Sci       Date:  1974-05       Impact factor: 5.285
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  92 in total

1.  Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis.

Authors:  P A Levin; I G Kurtser; A D Grossman
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-17       Impact factor: 11.205

2.  Novel filaments 5 nm in diameter constitute the cytosolic ring of the plastid division apparatus.

Authors:  S Miyagishima ; M Takahara; T Kuroiwa
Journal:  Plant Cell       Date:  2001-03       Impact factor: 11.277

3.  Direct interaction between the cell division protein FtsZ and the cell differentiation protein SpoIIE.

Authors:  I Lucet; A Feucht; M D Yudkin; J Errington
Journal:  EMBO J       Date:  2000-04-03       Impact factor: 11.598

4.  On the origin of branches in Escherichia coli.

Authors:  B Gullbrand; T Akerlund; K Nordström
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

5.  Straight and curved conformations of FtsZ are regulated by GTP hydrolysis.

Authors:  C Lu; M Reedy; H P Erickson
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

6.  Tubulin-like protofilaments in Ca2+-induced FtsZ sheets.

Authors:  J Löwe; L A Amos
Journal:  EMBO J       Date:  1999-05-04       Impact factor: 11.598

7.  Plastid division is driven by a complex mechanism that involves differential transition of the bacterial and eukaryotic division rings.

Authors:  M Takahara; T Mori; H Kuroiwa; T Higashiyama; T Kuroiwa
Journal:  Plant Cell       Date:  2001-10       Impact factor: 11.277

8.  Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast.

Authors:  D H Edwards; H B Thomaides; J Errington
Journal:  EMBO J       Date:  2000-06-01       Impact factor: 11.598

9.  In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function.

Authors:  Jesse Stricker; Harold P Erickson
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490

10.  FtsZ exhibits rapid movement and oscillation waves in helix-like patterns in Escherichia coli.

Authors:  Swapna Thanedar; William Margolin
Journal:  Curr Biol       Date:  2004-07-13       Impact factor: 10.834
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