Literature DB >> 8015713

Neurons derived from embryonal carcinoma (P19) cells express multiple GABAA receptor subunits and fully functional GABAA receptors.

J N Reynolds1, P J Ryan, A Prasad, G D Paterno.   

Abstract

The embryonal carcinoma (EC) cell line P19 can be induced to differentiate into neurons and glia by treatment with retinoic acid. Neuronal cells derived from P19 stem cells were found to express messenger RNAs for alpha, beta, and gamma 2 subunits of the GABAA receptor-chloride channel complex. Whole-cell voltage-clamp recording in differentiated P19 cells revealed that these cells possess GABA receptor-activated chloride currents which are blocked by bicuculline and potentiated by flurazepam. P19 EC cells thus represent a stable neuronal cell line which expresses functional GABAA receptors with all of the characteristics of native GABAA receptors.

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Year:  1994        PMID： 8015713     DOI： 10.1016/0304-3940(94)90726-9

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN： 0304-3940            Impact factor:   3.046


  3 in total

Review 1.  Neurotransmitter receptor expression and activity during neuronal differentiation of embryonal carcinoma and stem cells: from basic research towards clinical applications.

Authors:  H Ulrich; P Majumder
Journal:  Cell Prolif       Date:  2006-08       Impact factor: 6.831

2.  13-cis-retinoic acid suppresses hippocampal cell division and hippocampal-dependent learning in mice.

Authors:  James Crandall; Yasuo Sakai; Jinghua Zhang; Omanand Koul; Yann Mineur; Wim E Crusio; Peter McCaffery
Journal:  Proc Natl Acad Sci U S A       Date:  2004-03-29       Impact factor: 11.205

3.  Akting up in the GABA hypothesis of schizophrenia: Akt1 deficiency modulates GABAergic functions and hippocampus-dependent functions.

Authors:  Chia-Yuan Chang; Yi-Wen Chen; Tsu-Wei Wang; Wen-Sung Lai
Journal:  Sci Rep       Date:  2016-09-12       Impact factor: 4.379
  3 in total

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