Literature DB >> 8010976

Inhibition of calmodulin-dependent myosin light-chain kinase by growth-hormone-releasing factor and vasoactive intestinal peptide.

H Shiraga1, D Stallwood, M Ebadi, R Pfeiffer, D Landers, S Paul.   

Abstract

In view of the ability of calmodulin to bind vasoactive intestinal peptide (VIP) and growth-hormone-releasing factor (GRF) with high affinity [Stallwood, Brugger, Baggenstoss, Stemmer, Shiraga, Landers and Paul (1992) J. Biol. Chem. 267, 19617-19621], the effects of these neuropeptides on a model calmodulin-dependent enzyme, myosin light-chain kinase (MLCK), were studied. Both peptides were potent inhibitors of MLCK activity. The inhibition of enzyme activity by VIP and GRF was progressively overcome with increasing calmodulin concentrations, with no inhibition observed at a saturating calmodulin concentration. Nanomolar concentrations of MLCK blocked the formation of calmodulin-[125I-Tyr10]VIP complexes. These data provide support for a functional role of VIP and GRF binding by calmodulin.

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Year:  1994        PMID： 8010976      PMCID： PMC1138250          DOI： 10.1042/bj3000901

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  36 in total

1.  Identity of a membrane-bound vasoactive intestinal peptide-binding protein with calmodulin.

Authors:  D Stallwood; C H Brugger; B A Baggenstoss; P M Stemmer; H Shiraga; D F Landers; S Paul
Journal:  J Biol Chem       Date:  1992-09-25       Impact factor: 5.157

2.  Transfer of the hormone receptor for vaso-intestinal peptide to an adenylate cyclase system in another cell.

Authors:  M Laburthe; G Rosselin; M Rousset; A Zweibaum; M Korner; Z Selinger; M Schramm
Journal:  FEBS Lett       Date:  1979-02-01       Impact factor: 4.124

3.  1H NMR analysis and in vitro bioactivity of Leu27-bGRF(1-29)NH2 and its D-Ala2 and des-(Tyr1-Ala2)-analogs.

Authors:  D A Kloosterman; T A Scahill; R M Hillman; D L Cleary; T M Kubiak
Journal:  Pept Res       Date:  1991 Mar-Apr

Review 4.  Vasoactive intestinal peptide: its interactions with calmodulin and catalytic antibodies.

Authors:  S Paul; M Ebadi
Journal:  Neurochem Int       Date:  1993-09       Impact factor: 3.921

5.  Binding of protein kinase substrates by fluorescently labeled calmodulin.

Authors:  D A Malencik; T S Huang; S R Anderson
Journal:  Biochem Biophys Res Commun       Date:  1982-09-16       Impact factor: 3.575

Review 6.  Calmodulin plays a pivotal role in cellular regulation.

Authors:  W Y Cheung
Journal:  Science       Date:  1980-01-04       Impact factor: 47.728

7.  Agonist and antagonist properties of calmodulin fragments.

Authors:  D L Newton; M D Oldewurtel; M H Krinks; J Shiloach; C B Klee
Journal:  J Biol Chem       Date:  1984-04-10       Impact factor: 5.157

8.  Binding of hormones and neuropeptides by calmodulin.

Authors:  D A Malencik; S R Anderson
Journal:  Biochemistry       Date:  1983-04-12       Impact factor: 3.162

9.  Multiplicity of receptors for vasoactive intestinal polypeptide (VIP): differential effects of apamin on binding in brain, uterus and liver.

Authors:  J Fahrenkrug; S Gammeltoft; P Staun-Olsen; B Ottesen; A Sjöquist
Journal:  Peptides       Date:  1983 Mar-Apr       Impact factor: 3.750

10.  Calmodulin activates the isolated catalytic unit of brain adenylate cyclase.

Authors:  R S Salter; M H Krinks; C B Klee; E J Neer
Journal:  J Biol Chem       Date:  1981-10-10       Impact factor: 5.157
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  1 in total

Review 1.  PEST sequences in calmodulin-binding proteins.

Authors:  J A Barnes; A V Gomes
Journal:  Mol Cell Biochem       Date:  1995 Aug-Sep       Impact factor: 3.396
  1 in total

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