Literature DB >> 800621

Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching.

K Jacobson, Z Derzko, E S Wu, Y Hou, G Poste.   

Abstract

The use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin-receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin receptor (WGA-receptor) complexes on the surface of human embryo fibroblasts are mobile. These WGS-receptor complexes diffuse laterally (as opposed to flow) on the cell surface with a diffusion coefficient in the range of 2 X 10(-11) to 2 X 10(-10) cm2/sec. Both the percentage of mobile WGA-receptor complexes and the mean diffusion coefficient of these complexes are higher than that obtained from earlier FRAP measurements of the mobility of concanavalin A-receptor (Con A-receptor) complexes in a variety of cell types. The possible reasons for the differing mobilities of WGA and Con A receptors are discussed.

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Year:  1976        PMID： 800621     DOI： 10.1002/jss.400050411

Source DB:  PubMed          Journal:  J Supramol Struct        ISSN： 0091-7419


  73 in total

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