W Conca1, F Willmroth. 1. Department of Rheumatology and Clinical Immunology, University of Freiburg Medical School, Germany.
Abstract
OBJECTIVE: T lymphocytes are known to interact with cellular and structural components of the extracellular matrix. We investigated whether T lymphocytes could also contribute to matrix breakdown by expression of a matrix metalloproteinase (MMP) gene. METHODS: Complementary DNA (cDNA) was synthesized from messenger RNA extracted from cultured peripheral blood T lymphocytes after exposure to phorbol myristate acetate and calcium ionophore A23187 and amplified by the polymerase chain reaction with primers derived from two conserved domains in MMP genes. RESULTS: An amplification product of 402 basepairs was generated and cloned; sequence analysis revealed identity to human stromelysin-2 cDNA. Using the amplified stromelysin-2 cDNA as a probe for Northern analyses, we detected a 1.8-kilobase transcript in stimulated T lymphocytes. CONCLUSION: T lymphocytes are a potential source of stromelysin-2 transcripts and may have a role in the degradation of extracellular matrix constituents.
OBJECTIVE: T lymphocytes are known to interact with cellular and structural components of the extracellular matrix. We investigated whether T lymphocytes could also contribute to matrix breakdown by expression of a matrix metalloproteinase (MMP) gene. METHODS: Complementary DNA (cDNA) was synthesized from messenger RNA extracted from cultured peripheral blood T lymphocytes after exposure to phorbol myristate acetate and calcium ionophore A23187 and amplified by the polymerase chain reaction with primers derived from two conserved domains in MMP genes. RESULTS: An amplification product of 402 basepairs was generated and cloned; sequence analysis revealed identity to humanstromelysin-2 cDNA. Using the amplified stromelysin-2 cDNA as a probe for Northern analyses, we detected a 1.8-kilobase transcript in stimulated T lymphocytes. CONCLUSION: T lymphocytes are a potential source of stromelysin-2 transcripts and may have a role in the degradation of extracellular matrix constituents.
Authors: Rodolfo D Vicetti Miguel; Stephen A K Harvey; William A LaFramboise; Seth D Reighard; Dean B Matthews; Thomas L Cherpes Journal: PLoS One Date: 2013-03-13 Impact factor: 3.240
Authors: N Kayagaki; A Kawasaki; T Ebata; H Ohmoto; S Ikeda; S Inoue; K Yoshino; K Okumura; H Yagita Journal: J Exp Med Date: 1995-12-01 Impact factor: 14.307