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Literature DB >> 8001116

Isolation of a protein that is essential for the first step of nuclear protein import.

D Görlich¹, S Prehn, R A Laskey, E Hartmann.

Abstract

We have purified a cytosolic protein from Xenopus eggs that is essential for selective protein import into the cell nucleus. The purified protein, named importin, promotes signal-dependent binding of karyophilic proteins to the nuclear envelope. We have cloned, sequenced, and expressed a corresponding cDNA. Importin shows 44% sequence identity with SRP1p, a protein associated with the yeast nuclear pore complex. Complete, signal-dependent import into HeLa nuclei can be reconstituted by combining importin purified from Xenopus eggs or expressed in E. coli with Ran/TC4. Evidence for additional stimulatory factors is provided.

Entities: Gene Species

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Year: 1994 PMID： 8001116 DOI： 10.1016/0092-8674(94)90067-1

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

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Cited

257 in total

1. Optical recording of signal-mediated protein transport through single nuclear pore complexes.

Authors: O Keminer; J P Siebrasse; K Zerf; R Peters
Journal: Proc Natl Acad Sci U S A Date: 1999-10-12 Impact factor: 11.205

Review 2. Localization of BRCA1 protein at the cellular level.

Authors: C R De Potter; E D Coene; V R Schelfhout
Journal: J Mammary Gland Biol Neoplasia Date: 1998-10 Impact factor: 2.673

3. RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro.

Authors: B Ossareh-Nazari; C Maison; B E Black; L Lévesque; B M Paschal; C Dargemont
Journal: Mol Cell Biol Date: 2000-07 Impact factor: 4.272

4. The C-terminal proline-rich tail of human immunodeficiency virus type 2 Vpx is necessary for nuclear localization of the viral preintegration complex in nondividing cells.

Authors: H A Pancio; N Vander Heyden; L Ratner
Journal: J Virol Date: 2000-07 Impact factor: 5.103

10. A nonconventional nuclear localization signal within the UL84 protein of human cytomegalovirus mediates nuclear import via the importin alpha/beta pathway.

Authors: Peter Lischka; Gabriele Sorg; Michael Kann; Michael Winkler; Thomas Stamminger
Journal: J Virol Date: 2003-03 Impact factor: 5.103

Isolation of a protein that is essential for the first step of nuclear protein import.

1. Optical recording of signal-mediated protein transport through single nuclear pore complexes.

Review 2. Localization of BRCA1 protein at the cellular level.

3. RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro.

4. The C-terminal proline-rich tail of human immunodeficiency virus type 2 Vpx is necessary for nuclear localization of the viral preintegration complex in nondividing cells.

5. Nuclear import of IkappaBalpha is accomplished by a ran-independent transport pathway.

6. beta-catenin can be transported into the nucleus in a Ran-unassisted manner.

Review 7. Transport into and out of the nucleus.

8. A novel function for the 90 kDa heat-shock protein (Hsp90): facilitating nuclear export of 60 S ribosomal subunits.

9. Nuclear pore complex is able to transport macromolecules with diameters of about 39 nm.

10. A nonconventional nuclear localization signal within the UL84 protein of human cytomegalovirus mediates nuclear import via the importin alpha/beta pathway.