Literature DB >> 8000503

Biosynthesis and secretion of mucin-related products in Hs746T gastric cancer cells.

S Sano1, K Okazaki, Y Yamamoto.   

Abstract

We have previously studied the biosynthesis and secretion of mucin in the normal human stomach and reported that the tetramer of the 60-kDa subunit of mucin core protein was synthesized and highly glycosylated, and that only high molecular weight mucin was secreted. In this study, we investigated the mucin-related products of a gastric cancer cell line (Hs746T). Analysis of intracellular and extracellular products immunoprecipitated with an anti-apomucin monoclonal antibody revealed that a 110-kDa protein, the dimer of the 55-kDa subunit, was synthesized and secreted. Tunicamycin treatment inhibited the secretion of the 110-kDa protein. These findings suggest that N-glycosylation may be involved in the secretory mechanism of the mucin-related product.

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Year:  1994        PMID： 8000503     DOI： 10.1007/BF02365437

Source DB:  PubMed          Journal:  J Gastroenterol        ISSN： 0944-1174            Impact factor:   7.527


  50 in total

1.  Characterization of mucin glycoprotein-specific translation products from swine and human trachea, pancreas and colon.

Authors:  S Sangadala; P Wallace; J Mendicino
Journal:  Mol Cell Biochem       Date:  1991-07-24       Impact factor: 3.396

2.  Production of MUC1 and MUC2 mucins by human tumor cell lines.

Authors:  P L Devine; G T Layton; B A Clark; G W Birrell; B G Ward; P X Xing; I F McKenzie
Journal:  Biochem Biophys Res Commun       Date:  1991-07-31       Impact factor: 3.575

3.  Mucin synthesis and secretion in relation to spontaneous differentiation of colon cancer cells in vitro.

Authors:  Y Niv; J C Byrd; S B Ho; R Dahiya; Y S Kim
Journal:  Int J Cancer       Date:  1992-01-02       Impact factor: 7.396

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Covalent oligomerization of rat gastric mucin occurs in the rough endoplasmic reticulum, is N-glycosylation-dependent, and precedes initial O-glycosylation.

Authors:  J Dekker; G J Strous
Journal:  J Biol Chem       Date:  1990-10-25       Impact factor: 5.157

6.  Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors.

Authors:  F Oyama; S Hirohashi; Y Shimosato; K Titani; K Sekiguchi
Journal:  J Biol Chem       Date:  1989-06-25       Impact factor: 5.157

7.  Immunohistochemistry of gastric carcinomas and associated diseases: novel distribution of carcinoembryonic antigen and secretory component on the surface of gastric cancer cells.

Authors:  H Nagura; Y Tsutsumi; Y Shioda; K Watanabe
Journal:  J Histochem Cytochem       Date:  1983-01       Impact factor: 2.479

8.  Glycoprotein nature of yeast alkaline phosphatase. Formation of active enzyme in the presence of tunicamycin.

Authors:  H R Onishi; J S Tkacz; J O Lampen
Journal:  J Biol Chem       Date:  1979-12-10       Impact factor: 5.157

9.  Interactions of misfolded influenza virus hemagglutinin with binding protein (BiP).

Authors:  S M Hurtley; D G Bole; H Hoover-Litty; A Helenius; C S Copeland
Journal:  J Cell Biol       Date:  1989-06       Impact factor: 10.539

10.  Synthesis and processing of molecules bearing thymus leukemia antigen.

Authors:  E Rothenberg; E A Boyse
Journal:  J Exp Med       Date:  1979-10-01       Impact factor: 14.307
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  1 in total

1.  Biliopancreatic fistula caused by an intraductal papillary-mucinous tumor of the pancreas confirmed by biochemical analysis of mucin.

Authors:  Shuichi Sano; Isao Nishimori; Nobuto Okamoto; Takuhiro Kohsaki; Saburo Onishi; Masanori Morita; Keijiro Araki
Journal:  Int J Gastrointest Cancer       Date:  2003
  1 in total

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