Literature DB >> 7999067

A rapid and efficient PCR-based method for synthesizing high-molecular-weight multimers of oligonucleotides.

F Hemat1, K McEntee.   

Abstract

We have developed a simple PCR based approach for synthesizing high-molecular-weight DNA multimers of short oligonucleotide elements in yields sufficient for preparing DNA affinity columns or for using as probes to detect sequence-specific DNA binding proteins. The technique is rapid, efficient and produces multimers consisting of 30-100 repeating units.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7999067     DOI： 10.1006/bbrc.1994.2690

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  miR-ID: a novel, circularization-based platform for detection of microRNAs.

Authors:  Pavan Kumar; Brian H Johnston; Sergei A Kazakov
Journal:  RNA       Date:  2010-12-17       Impact factor: 4.942

2.  Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae.

Authors:  A P Schmitt; K McEntee
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

3.  Regulation of the p53 transcriptional response by structurally diverse core promoters.

Authors:  José M Morachis; Christopher M Murawsky; Beverly M Emerson
Journal:  Genes Dev       Date:  2009-12-29       Impact factor: 11.361

4.  Functional analysis of the promoter of the mitochondrial phosphate carrier human gene: identification of activator and repressor elements and their transcription factors.

Authors:  Vito Iacobazzi; Vittoria Infantino; Paola Costanzo; Paola Izzo; Ferdinando Palmieri
Journal:  Biochem J       Date:  2005-11-01       Impact factor: 3.857

5.  Initiator recognition in a primitive eukaryote: IBP39, an initiator-binding protein from Trichomonas vaginalis.

Authors:  D R Liston; A O Lau; D Ortiz; S T Smale; P J Johnson
Journal:  Mol Cell Biol       Date:  2001-11       Impact factor: 4.272

6.  The trans-acting protein interacting with the DNA motif proximal to the transcriptional start site of plant L-asparaginase is bacterial sarcosine oxidase.

Authors:  William T Jones; Taha Al-Samarrai; Janice M Reeves; Gordon B Ryan; Christopher A Kirk; Eva Vincze; Dawn Harvey; Marie McCambridge; David Greenwood; Paul H S Reynolds
Journal:  J Bacteriol       Date:  2004-02       Impact factor: 3.490

7.  Transcriptional enhancer factor 1 (TEF-1/TEAD1) mediates activation of IFITM3 gene by BRGl.

Authors:  Suresh Cuddapah; Kairong Cui; Keji Zhao
Journal:  FEBS Lett       Date:  2008-01-03       Impact factor: 4.124
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.