Literature DB >> 799614

A hot wire device for cutting tissue culture flasks.

S W McKelvey, P A Singer, J H Lillie, D K MacCallum.   

Abstract

A small hot wire device for cutting plastic culture was can be constructed of steel rod, brass screws, nichrome wire and acrylic plastic sheeting and tubing. The nichrome wire is heated using a variable power transformer. Four sequential cuts are made in the culture flask bottom and the bottom separated from the remainder of the flask. Cultures can be stained, air-dried and cover slips affixed with PVP or epoxy resin. This method of cutting culture ware avoids the formation of small bits of polystyrene generated by rotating discs or saws.

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Year:  1976        PMID: 799614     DOI: 10.1007/bf02796370

Source DB:  PubMed          Journal:  In Vitro        ISSN: 0073-5655


  3 in total

1.  Rapid staining and preservation of cells grown in plastic tissue culture flasks.

Authors:  C H Fox; M Cottler-Fox; K K Sanford
Journal:  Stain Technol       Date:  1974-03

2.  Advantages of epoxy resin as a mounting medium for light microscopy.

Authors:  R O Ores
Journal:  Stain Technol       Date:  1971-11

3.  A study of sieve element starch using sequential enzymatic digestion and electron microscopy.

Authors:  B A Palevitz; E H Newcomb
Journal:  J Cell Biol       Date:  1970-05       Impact factor: 10.539

  3 in total
  1 in total

1.  Cultured neonatal rat oligodendrocytes are enriched in acid hydrolase activities.

Authors:  E J Bradel; H R Sloan
Journal:  Neurochem Res       Date:  1988-10       Impact factor: 3.996

  1 in total

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