| Literature DB >> 7990145 |
A K Basak1, D I Stuart, T Nikura, D H Bishop, D C Kelly, A Fearn, R W Titball.
Abstract
Alpha-toxin of Clostridium perfringens, cloned in Escherichia coli, has been purified and crystallized from ammonium sulphate using the hanging drop vapour diffusion method at 20 degrees C. The crystals diffract to a minimum Bragg spacing of 2.7 A, belong to the space group R32 (with a = b = 153.3 A, c = 95.4 A, alpha = beta = 90 degrees and gamma = 120 degrees) and contain a single polypeptide chain in the crystallographic unit.Entities:
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Year: 1994 PMID： 7990145 DOI： 10.1006/jmbi.1994.1758
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469