Literature DB >> 7989563

Effects of enhancing agents on detection of cytomegalovirus in clinical specimens.

K St George1, C R Rinaldo.   

Abstract

Dimethyl sulfoxide, dexamethasone, and calcium were tested in combination for their enhancing effects on cytomegalovirus detection in shell vial cultures on 1,579 clinical specimens obtained primarily from adult solid-organ transplant recipients. Fluorescent-focus counts were elevated for the cytomegalovirus-positive urine specimens (P < 0.01) and throat washings (P < 0.05) but not for the tissue biopsy or blood samples. Epidermal growth factor also increased focus counts but provided no additional benefit when used in combination with the other agents. The triple-combination treatment did not increase the number of positive specimens identified.

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Year:  1994        PMID： 7989563      PMCID： PMC263925          DOI： 10.1128/jcm.32.8.2024-2027.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

1.  Rapid diagnosis of invasive cytomegalovirus infection by examination of tissue specimens in centrifugation culture.

Authors:  C A Gleaves; E C Reed; R C Hackman; J D Meyers
Journal:  Am J Clin Pathol       Date:  1987-09       Impact factor: 2.493

2.  Physiological state of human embryonic lung cells affects their response to human cytomegalovirus.

Authors:  J M DeMarchi; A S Kaplan
Journal:  J Virol       Date:  1977-07       Impact factor: 5.103

3.  Alteration of the growth of cytomegalovirus and herpes simplex virus type 1 by epidermal growth factor, a contaminant of crude human chorionic gonadotropin preparations.

Authors:  G E Knox; D W Reynolds; S Cohen; C A Alford
Journal:  J Clin Invest       Date:  1978-06       Impact factor: 14.808

4.  Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity.

Authors:  J B Hudson; V Misra; T R Mosmann
Journal:  Virology       Date:  1976-07-01       Impact factor: 3.616

5.  Immediate early antigens in human cytomegalovirus infected cells.

Authors:  S Michelson-Fiske; F Horodniceanu; J C Guillon
Journal:  Nature       Date:  1977-12-15       Impact factor: 49.962

6.  Rapid detection of cytomegalovirus by tissue culture, centrifugation, and immunofluorescence with a monoclonal antibody to an early nuclear antigen.

Authors:  G M Thiele; M S Bicak; A Young; J Kinsey; R J White; D T Purtilo
Journal:  J Virol Methods       Date:  1987-07       Impact factor: 2.014

7.  Comparison of two rapid culture methods for detection of cytomegalovirus in clinical specimens.

Authors:  P D Swenson; M H Kaplan
Journal:  J Clin Microbiol       Date:  1987-12       Impact factor: 5.948

8.  Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci.

Authors:  P D Griffiths; D D Panjwani; P R Stirk; M G Ball; M Ganczakowski; H A Blacklock; H G Prentice
Journal:  Lancet       Date:  1984-12-01       Impact factor: 79.321

9.  Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.

Authors:  C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

10.  Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone for detection of cytomegalovirus.

Authors:  M J Espy; A D Wold; D M Ilstrup; T F Smith
Journal:  J Clin Microbiol       Date:  1988-06       Impact factor: 5.948
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  2 in total

1.  Glucocorticosteroids trigger reactivation of human cytomegalovirus from latently infected myeloid cells and increase the risk for HCMV infection in D+R+ liver transplant patients.

Authors:  Ellen Van Damme; Sarah Sauviller; Betty Lau; Bart Kesteleyn; Paul Griffiths; Andrew Burroughs; Vincent Emery; John Sinclair; Marnix Van Loock
Journal:  J Gen Virol       Date:  2014-10-13       Impact factor: 3.891

2.  Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections.

Authors:  Sonali K Sanghavi; Arlene Bullotta; Shahid Husain; Charles R Rinaldo
Journal:  J Med Virol       Date:  2011-11-03       Impact factor: 2.327
  2 in total

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