Literature DB >> 7988713

Sensory rhodopsin I photocycle intermediate SRI380 contains 13-cis retinal bound via an unprotonated Schiff base.

U Haupts1, W Eisfeld, M Stockburger, D Oesterhelt.   

Abstract

Sensory rhodopsin I (SRI), the mutated derivative SRI-D76N and the complex of SRI with its transducer HtrI were overexpressed in Halobacterium salinarium and analyzed by resonance Raman spectroscopy. In the initial state SRI contains all-trans retinal bound via a protonated Schiff base as confirmed by retinal extraction which yields 95 +/- 3% all-trans retinal. The photocycle intermediate absorbing maximally at 380 nm (SRI380) contains a Schiff base linkage between the protein and 13-cis retinal. Extraction of illuminated SRI yields up to 93% 13-cis retinal. Neither the mutation D76N nor HtrI changed the vibrational pattern of the chromophore.

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Year:  1994        PMID： 7988713     DOI： 10.1016/0014-5793(94)01226-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

Review 1.  Bioenergetics of the Archaea.

Authors:  G Schäfer; M Engelhard; V Müller
Journal:  Microbiol Mol Biol Rev       Date:  1999-09       Impact factor: 11.056

2.  Time-resolved absorption and photothermal measurements with sensory rhodopsin I from Halobacterium salinarum.

Authors:  A Losi; S E Braslavsky; W Gärtner; J L Spudich
Journal:  Biophys J       Date:  1999-04       Impact factor: 4.033

3.  Electric-field dependent decays of two spectroscopically different M-states of photosensory rhodopsin II from Natronobacterium pharaonis.

Authors:  Laura Rivas; Silke Hippler-Mreyen; Martin Engelhard; Peter Hildebrandt
Journal:  Biophys J       Date:  2003-06       Impact factor: 4.033

4.  Protonatable residues at the cytoplasmic end of transmembrane helix-2 in the signal transducer HtrI control photochemistry and function of sensory rhodopsin I.

Authors:  K H Jung; J L Spudich
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-25       Impact factor: 11.205

5.  A Schiff base connectivity switch in sensory rhodopsin signaling.

Authors:  Oleg A Sineshchekov; Jun Sasaki; Brian J Phillips; John L Spudich
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-13       Impact factor: 11.205

6.  Sensory rhodopsin-I as a bidirectional switch: opposite conformational changes from the same photoisomerization.

Authors:  Jun Sasaki; Hazuki Takahashi; Yuji Furutani; Hideki Kandori; John L Spudich
Journal:  Biophys J       Date:  2011-05-04       Impact factor: 4.033

7.  Different modes of proton translocation by sensory rhodopsin I.

Authors:  U Haupts; E Bamberg; D Oesterhelt
Journal:  EMBO J       Date:  1996-04-15       Impact factor: 11.598

8.  His166 is critical for active-site proton transfer and phototaxis signaling by sensory rhodopsin I.

Authors:  X N Zhang; J L Spudich
Journal:  Biophys J       Date:  1997-09       Impact factor: 4.033

9.  The photoreceptor sensory rhodopsin I as a two-photon-driven proton pump.

Authors:  U Haupts; C Haupts; D Oesterhelt
Journal:  Proc Natl Acad Sci U S A       Date:  1995-04-25       Impact factor: 11.205
  9 in total

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