Literature DB >> 7987390

Human artificial episomal chromosomes for cloning large DNA fragments in human cells.

T Q Sun1, D A Fenstermacher, J M Vos.   

Abstract

We have developed a human artificial episomal chromosome (HAEC) system, based on the latent replication origin of the large herpes Epstein-Barr virus, for the propagation and stable maintenance of DNA as circular minichromosomes in human cells. Individual HAECs carried human genomic inserts ranging from 60-330 kb and appeared genetically stable. An HAEC library of 1,500 independent clones carrying random human genomic fragments with average sizes of 150-200 kb was established and allowed recovery of the HAEC DNA. Our autologous HAEC system, with human DNA cloned directly in human cells, provides an important tool for functional study of large mammalian DNA regions and gene therapy.

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Year:  1994        PMID： 7987390     DOI： 10.1038/ng0994-33

Source DB:  PubMed          Journal:  Nat Genet        ISSN： 1061-4036            Impact factor:   38.330


  17 in total

1.  An adenovirus-Epstein-Barr virus hybrid vector that stably transforms cultured cells with high efficiency.

Authors:  B T Tan; L Wu; A J Berk
Journal:  J Virol       Date:  1999-09       Impact factor: 5.103

2.  A Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 ORF50 deletion mutant is defective for reactivation of latent virus and DNA replication.

Authors:  Yiyang Xu; David P AuCoin; Alicia Rodriguez Huete; Sylvia A Cei; Lisa J Hanson; Gregory S Pari
Journal:  J Virol       Date:  2005-03       Impact factor: 5.103

3.  Stable episomal maintenance of yeast artificial chromosomes in human cells.

Authors:  K Simpson; A McGuigan; C Huxley
Journal:  Mol Cell Biol       Date:  1996-09       Impact factor: 4.272

4.  Modification of bacterial artificial chromosome clones using Cre recombinase: introduction of selectable markers for expression in eukaryotic cells.

Authors:  S Y Kim; S K Horrigan; J L Altenhofen; Z H Arbieva; R Hoffman; C A Westbrook
Journal:  Genome Res       Date:  1998-04       Impact factor: 9.043

5.  Isolating large nested deletions in bacterial and P1 artificial chromosomes by in vivo P1 packaging of products of Cre-catalysed recombination between the endogenous and a transposed loxP site.

Authors:  P K Chatterjee; J S Coren
Journal:  Nucleic Acids Res       Date:  1997-06-01       Impact factor: 16.971

6.  Efficient male and female germline transmission of a human chromosomal vector in mice.

Authors:  T Voet; J Vermeesch; A Carens; J Dürr; C Labaere; H Duhamel; G David; P Marynen
Journal:  Genome Res       Date:  2001-01       Impact factor: 9.043

7.  Stability of a functional murine satellite DNA-based artificial chromosome across mammalian species.

Authors:  H Telenius; A Szeles; J Keresö; E Csonka; T Praznovszky; S Imreh; A Maxwell; C F Perez; J I Drayer; G Hadlaczky
Journal:  Chromosome Res       Date:  1999       Impact factor: 5.239

8.  Bacterial artificial chromosomes establish replication timing and sub-nuclear compartment de novo as extra-chromosomal vectors.

Authors:  Jiao Sima; Daniel A Bartlett; Molly R Gordon; David M Gilbert
Journal:  Nucleic Acids Res       Date:  2018-02-28       Impact factor: 16.971

9.  Initiation of latent DNA replication in the Epstein-Barr virus genome can occur at sites other than the genetically defined origin.

Authors:  R D Little; C L Schildkraut
Journal:  Mol Cell Biol       Date:  1995-05       Impact factor: 4.272

10.  Regulated expression of a transgene introduced on an oriP/EBNA-1 PAC shuttle vector into human cells.

Authors:  Hanne A Askautrud; Elisabet Gjernes; Gro L Størvold; Mona M Lindeberg; Jim Thorsen; Hans Prydz; Eirik Frengen
Journal:  BMC Biotechnol       Date:  2009-10-16       Impact factor: 2.563
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