Literature DB >> 7987245

Application of spheroid culture to human hepatocytes and maintenance of their differentiation.

J Z Tong, S Sarrazin, D Cassio, F Gauthier, F Alvarez.   

Abstract

Human hepatocytes cultured with a hormonally defined medium on non-adherent poly-(2-hydroxyethyl methacrylate)-coated surface were able to form spheroids. The maintenance of liver-specific functions was assessed by following secretion of albumin, transferrin and alpha 1-antitrypsin that were still detectable after 4 months of spheroidal culture. Moreover, cytochrome P-450 IA was induced by methylcholanthrene for up to 2 weeks. This cell system is very promising for long-term in vitro studies of human hepatocyte functions.

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Year:  1994        PMID： 7987245     DOI： 10.1016/0248-4900(94)90058-2

Source DB:  PubMed          Journal:  Biol Cell        ISSN： 0248-4900            Impact factor:   4.458


  15 in total

1.  Microgravity as a means to incorporate HepG2 aggregates in polysaccharide-protein hybrid scaffold.

Authors:  P R Sarika; Nirmala Rachel James; P R Anilkumar; Deepa K Raj; T V Kumary
Journal:  J Mater Sci Mater Med       Date:  2015-12-24       Impact factor: 3.896

2.  Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting.

Authors:  Xuanyi Ma; Xin Qu; Wei Zhu; Yi-Shuan Li; Suli Yuan; Hong Zhang; Justin Liu; Pengrui Wang; Cheuk Sun Edwin Lai; Fabian Zanella; Gen-Sheng Feng; Farah Sheikh; Shu Chien; Shaochen Chen
Journal:  Proc Natl Acad Sci U S A       Date:  2016-02-08       Impact factor: 11.205

Review 3.  Biomaterial strategies for stem cell maintenance during in vitro expansion.

Authors:  Xiang-Zhen Yan; Jeroen J J P van den Beucken; Sanne K Both; Pi-Shan Yang; John A Jansen; Fang Yang
Journal:  Tissue Eng Part B Rev       Date:  2013-12-05       Impact factor: 6.389

4.  Multilayer hepatocyte aggregates on porous expanded polytetrafluoroethylene.

Authors:  T Tokiwa; M Kodama
Journal:  In Vitro Cell Dev Biol Anim       Date:  1997-03       Impact factor: 2.416

Review 5.  Emerging In Vitro Liver Technologies for Drug Metabolism and Inter-Organ Interactions.

Authors:  Shyam Sundhar Bale; Laura Moore; Martin Yarmush; Rohit Jindal
Journal:  Tissue Eng Part B Rev       Date:  2016-06-01       Impact factor: 6.389

6.  Multilayer rat hepatocyte aggregates formed on expanded polytetrafluoroethylene surface.

Authors:  T Tokiwa; J Kano; M Kodama; T Matsumura
Journal:  Cytotechnology       Date:  1997-11       Impact factor: 2.058

7.  Dynamic interplay of flow and collagen stabilizes primary hepatocytes culture in a microfluidic platform.

Authors:  Manjunath Hegde; Rohit Jindal; Abhinav Bhushan; Shyam Sundhar Bale; William J McCarty; Inna Golberg; O Berk Usta; Martin L Yarmush
Journal:  Lab Chip       Date:  2014-04-28       Impact factor: 6.799

8.  Engraftment potential of spheroid-forming hepatic endoderm derived from human embryonic stem cells.

Authors:  Sung-Eun Kim; Su Yeon An; Dong-Hun Woo; Jiyou Han; Jong Hyun Kim; Yu Jin Jang; Jeong Sang Son; Hyunwon Yang; Yong Pil Cheon; Jong-Hoon Kim
Journal:  Stem Cells Dev       Date:  2013-03-12       Impact factor: 3.272

9.  Maintenance of liver functions in rat hepatocytes cultured as spheroids in a rotating wall vessel.

Authors:  Lanika A Brown; Linda M Arterburn; Ana P Miller; Nancy L Cowger; Sonya M Hartley; Annette Andrews; Paul M Silber; Albert P Li
Journal:  In Vitro Cell Dev Biol Anim       Date:  2003 Jan-Feb       Impact factor: 2.416

Review 10.  Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.

Authors:  Patricio Godoy; Nicola J Hewitt; Ute Albrecht; Melvin E Andersen; Nariman Ansari; Sudin Bhattacharya; Johannes Georg Bode; Jennifer Bolleyn; Christoph Borner; Jan Böttger; Albert Braeuning; Robert A Budinsky; Britta Burkhardt; Neil R Cameron; Giovanni Camussi; Chong-Su Cho; Yun-Jaie Choi; J Craig Rowlands; Uta Dahmen; Georg Damm; Olaf Dirsch; María Teresa Donato; Jian Dong; Steven Dooley; Dirk Drasdo; Rowena Eakins; Karine Sá Ferreira; Valentina Fonsato; Joanna Fraczek; Rolf Gebhardt; Andrew Gibson; Matthias Glanemann; Chris E P Goldring; María José Gómez-Lechón; Geny M M Groothuis; Lena Gustavsson; Christelle Guyot; David Hallifax; Seddik Hammad; Adam Hayward; Dieter Häussinger; Claus Hellerbrand; Philip Hewitt; Stefan Hoehme; Hermann-Georg Holzhütter; J Brian Houston; Jens Hrach; Kiyomi Ito; Hartmut Jaeschke; Verena Keitel; Jens M Kelm; B Kevin Park; Claus Kordes; Gerd A Kullak-Ublick; Edward L LeCluyse; Peng Lu; Jennifer Luebke-Wheeler; Anna Lutz; Daniel J Maltman; Madlen Matz-Soja; Patrick McMullen; Irmgard Merfort; Simon Messner; Christoph Meyer; Jessica Mwinyi; Dean J Naisbitt; Andreas K Nussler; Peter Olinga; Francesco Pampaloni; Jingbo Pi; Linda Pluta; Stefan A Przyborski; Anup Ramachandran; Vera Rogiers; Cliff Rowe; Celine Schelcher; Kathrin Schmich; Michael Schwarz; Bijay Singh; Ernst H K Stelzer; Bruno Stieger; Regina Stöber; Yuichi Sugiyama; Ciro Tetta; Wolfgang E Thasler; Tamara Vanhaecke; Mathieu Vinken; Thomas S Weiss; Agata Widera; Courtney G Woods; Jinghai James Xu; Kathy M Yarborough; Jan G Hengstler
Journal:  Arch Toxicol       Date:  2013-08-23       Impact factor: 5.153
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