Literature DB >> 7986055

A transcriptional reporter for in vivo promoter analysis in the archaeon Haloferax volcanii.

J R Palmer1, C J Daniels.   

Abstract

We have used a modified intron-containing tRNA(Pro(UGG) gene (tRNA(ProM), derived from the Saccharomyces cerevisiae tRNA(Pro(UGG) gene, as a reporter to measure in vivo transcription from a halophilic archaeon promoter. Coupling of the yeast tRNA(ProM) gene to the Haloferax volcanii tRNA(Lys) promoter on the H. volcanii plasmid pWL201 led to the production of a single stable transcript that was readily quantitated by Northern (RNA) blot analysis. Comparison of tRNA(ProM) RNA production from constructs containing the wild-type tRNA(Lys) promoter and those containing mutant tRNA(Lys) promoters demonstrated that this assay system can be used to measure expression from strong and weak promoters.

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Year:  1994        PMID： 7986055      PMCID： PMC201898          DOI： 10.1128/aem.60.10.3867-3869.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  TFIIB, an evolutionary link between the transcription machineries of archaebacteria and eukaryotes.

Authors:  C Ouzounis; C Sander
Journal:  Cell       Date:  1992-10-16       Impact factor: 41.582

2.  The RNA component of RNase P from the archaebacterium Haloferax volcanii.

Authors:  D T Nieuwlandt; E S Haas; C J Daniels
Journal:  J Biol Chem       Date:  1991-03-25       Impact factor: 5.157

3.  PCR amplification of long DNA fragments.

Authors:  M R Ponce; J L Micol
Journal:  Nucleic Acids Res       Date:  1992-02-11       Impact factor: 16.971

4.  Elements of an archaeal promoter defined by mutational analysis.

Authors:  J Hain; W D Reiter; U Hüdepohl; W Zillig
Journal:  Nucleic Acids Res       Date:  1992-10-25       Impact factor: 16.971

5.  The TATA-binding protein: a general transcription factor in eukaryotes and archaebacteria.

Authors:  T Rowlands; P Baumann; S P Jackson
Journal:  Science       Date:  1994-05-27       Impact factor: 47.728

6.  Putative tfIIs gene of Sulfolobus acidocaldarius encoding an archaeal transcription elongation factor is situated directly downstream of the gene for a small subunit of DNA-dependent RNA polymerase.

Authors:  D Langer; W Zillig
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

7.  Complete nucleotide sequence of an archaeal (Pyrococcus woesei) gene encoding a homolog of eukaryotic transcription factor IIB (TFIIB).

Authors:  R Creti; P Londei; P Cammarano
Journal:  Nucleic Acids Res       Date:  1993-06-25       Impact factor: 16.971

8.  Mutational analysis of an archaebacterial promoter: essential role of a TATA box for transcription efficiency and start-site selection in vitro.

Authors:  W D Reiter; U Hüdepohl; W Zillig
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

9.  An expression vector for the archaebacterium Haloferax volcanii.

Authors:  D T Nieuwlandt; C J Daniels
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

10.  Control regions of an archaeal gene. A TATA box and an initiator element promote cell-free transcription of the tRNA(Val) gene of Methanococcus vannielii.

Authors:  W Hausner; G Frey; M Thomm
Journal:  J Mol Biol       Date:  1991-12-05       Impact factor: 5.469
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  4 in total

1.  Halophilic 20S proteasomes of the archaeon Haloferax volcanii: purification, characterization, and gene sequence analysis.

Authors:  H L Wilson; H C Aldrich; J Maupin-Furlow
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  Characterization of two heat shock genes from Haloferax volcanii: a model system for transcription regulation in the Archaea.

Authors:  Y P Kuo; D K Thompson; A St Jean; R L Charlebois; C J Daniels
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

3.  In vivo definition of an archaeal promoter.

Authors:  J R Palmer; C J Daniels
Journal:  J Bacteriol       Date:  1995-04       Impact factor: 3.490

4.  Coenzyme F390 synthetase from Methanobacterium thermoautotrophicum Marburg belongs to the superfamily of adenylate-forming enzymes.

Authors:  P Vermeij; R J van der Steen; J T Keltjens; G D Vogels; T Leisinger
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490
  4 in total

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