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Literature DB >> 7981941

Ultrastructural detection of kinetochores by silver impregnation.

J S Rufas¹, C Mazzella, C García de la Vega, J A Suja.

Abstract

We describe a simple silver impregnation method for the ultrastructural detection of kinetochores on meiotic chromosomes of the grasshopper Eyprepocnemis plorans. Testes were fixed with glutaraldehyde and silver-impregnated. After Epon 812 embedding, ultrathin cutting and counterstaining with uranyl acetate, sections were studied by transmission electron microscopy. The meiotic chromosomes showed differentially silver-impregnated 'ball and cup' kinetochores. Some pericentriolar material also showed silver deposits. These observations are discussed in the light of previous results obtained by light microscopy of silver-stained spermatocytes in which both kinetochores and pericentriolar material were also preferentially stained. These results suggest a role for acidic proteins in the composition of these structures.

Entities: Chemical Species

Mesh：

Year: 1994 PMID： 7981941 DOI： 10.1007/BF01552796

Source DB: PubMed Journal: Chromosome Res ISSN： 0967-3849 Impact factor: 5.239

24 in total

1. DNA topoisomerase II alpha is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2.

Authors: S Taagepera; P N Rao; F H Drake; G J Gorbsky
Journal: Proc Natl Acad Sci U S A Date: 1993-09-15 Impact factor: 11.205

2. New selective Giemsa technique for human chromosomes, Cd staining.

Authors: H Eiberg
Journal: Nature Date: 1974-03-01 Impact factor: 49.962

3. Cytochemical studies on chromosome ultrastructure.

Authors: W F Sheridan; R J Barrnett
Journal: J Ultrastruct Res Date: 1969-05

Review 4. The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber.

Authors: C L Rieder
Journal: Int Rev Cytol Date: 1982

5. A technique for the simultaneous staining of both nucleolar organizer regions and kinetochores of human chromosomes with silver.

Authors: T E Denton; W R Brooke; W M Howell
Journal: Stain Technol Date: 1977-11

6. Mapping DNA within the mammalian kinetochore.

Authors: C A Cooke; D P Bazett-Jones; W C Earnshaw; J B Rattner
Journal: J Cell Biol Date: 1993-03 Impact factor: 10.539

7. The centromere-kinetochore complex: a repeat subunit model.

Authors: R P Zinkowski; J Meyne; B R Brinkley
Journal: J Cell Biol Date: 1991-06 Impact factor: 10.539

8. Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.

Authors: G J Gorbsky; P J Sammak; G G Borisy
Journal: J Cell Biol Date: 1987-01 Impact factor: 10.539

Ultrastructural detection of kinetochores by silver impregnation.

1. DNA topoisomerase II alpha is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2.

2. New selective Giemsa technique for human chromosomes, Cd staining.

3. Cytochemical studies on chromosome ultrastructure.

Review 4. The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber.

5. A technique for the simultaneous staining of both nucleolar organizer regions and kinetochores of human chromosomes with silver.

6. Mapping DNA within the mammalian kinetochore.

7. The centromere-kinetochore complex: a repeat subunit model.

8. Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.

9. The motor for poleward chromosome movement in anaphase is in or near the kinetochore.

10. Ribonucleoprotein staining of centrioles and kinetochores in newt lung cell spindles.

1. Chromosomal strategies for adaptation to univalency.

2. A comparative study of orientation at behavior of univalent in living grasshopper spermatocytes.

3. Dynamics of cohesin subunits in grasshopper meiotic divisions.

4. Involvement of synaptonemal complex proteins in sex chromosome segregation during marsupial male meiosis.