Literature DB >> 7980527

Autocrine inhibition of the epidermal growth factor receptor by intracellular expression of a single-chain antibody.

R R Beerli1, W Wels, N E Hynes.   

Abstract

A gene encoding a single-chain antibody which specifically binds the human epidermal growth factor (EGF) receptor has been constructed and expressed intracellularly. The single-chain antibody is derived from monoclonal antibody 225 which competes with EGF for binding to the extracellular domain of the receptor. The single-chain antibody was provided with a signal peptide to direct it to the secretory pathway and was expressed in EGF receptor transformed NIH/3T3 fibroblasts. EGF induced activation of its receptor was reduced in these cells. In addition, EGF-induced anchorage-independent growth of the cells was inhibited. The data suggest that the single-chain antibody functions in an autocrine fashion to inhibit the activity of the EGF receptor.

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Year:  1994        PMID： 7980527     DOI： 10.1006/bbrc.1994.2511

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

1.  Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmic reticulum.

Authors:  Bhaswati Barat; Anna M Wu
Journal:  Biomol Eng       Date:  2007-02-15

2.  Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the alpha subunit of the receptor.

Authors:  J H Richardson; J G Sodroski; T A Waldmann; W A Marasco
Journal:  Proc Natl Acad Sci U S A       Date:  1995-04-11       Impact factor: 11.205

3.  Dysregulation of the cohesin subunit RAD21 by Hepatitis C virus mediates host-virus interactions.

Authors:  Shira Perez; Michael Gevor; Ateret Davidovich; Antony Kaspi; Katreena Yamin; Tom Domovich; Tomer Meirson; Avi Matityahu; Yehuda Brody; Salomon M Stemmer; Assam El-Osta; Izhak Haviv; Itay Onn; Meital Gal-Tanamy
Journal:  Nucleic Acids Res       Date:  2019-03-18       Impact factor: 16.971

4.  Inhibition of protease-inhibitor-resistant hepatitis C virus replicons and infectious virus by intracellular intrabodies.

Authors:  Meital Gal-Tanamy; Romy Zemel; Larissa Bachmatov; Rohit K Jangra; Assaf Shapira; Rodrigo A Villanueva; Minkyung Yi; Stanley M Lemon; Itai Benhar; Ran Tur-Kaspa
Journal:  Antiviral Res       Date:  2010-08-10       Impact factor: 5.970

5.  ErbB-1 and ErbB-2 acquire distinct signaling properties dependent upon their dimerization partner.

Authors:  M A Olayioye; D Graus-Porta; R R Beerli; J Rohrer; B Gay; N E Hynes
Journal:  Mol Cell Biol       Date:  1998-09       Impact factor: 4.272

Review 6.  Inhibition of signaling from Type 1 receptor tyrosine kinases via intracellular expression of single-chain antibodies.

Authors:  R R Beerli; W Wels; N E Hynes
Journal:  Breast Cancer Res Treat       Date:  1996       Impact factor: 4.872

7.  Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling.

Authors:  D Graus-Porta; R R Beerli; N E Hynes
Journal:  Mol Cell Biol       Date:  1995-03       Impact factor: 4.272

8.  Construction and functional characterization of scFv(14E1)-ETA - a novel, highly potent antibody-toxin specific for the EGF receptor.

Authors:  M Schmidt; E Vakalopoulou; D W Schneider; W Wels
Journal:  Br J Cancer       Date:  1997       Impact factor: 9.075

9.  Targeted inhibition of tumour cell growth by a bispecific single-chain toxin containing an antibody domain and TGF alpha.

Authors:  M Schmidt; W Wels
Journal:  Br J Cancer       Date:  1996-09       Impact factor: 9.075

Review 10.  Specific in vivo knockdown of protein function by intrabodies.

Authors:  Andrea L J Marschall; Stefan Dübel; Thomas Böldicke
Journal:  MAbs       Date:  2015-08-07       Impact factor: 5.857
  10 in total

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