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Literature DB >> 7980526

Cloning and expression of recombinant human growth/differentiation factor 5.

G Hötten¹, H Neidhardt, B Jacobowsky, J Pohl.

Abstract

The complete amino acid sequence of human Growth/differentiation factor 5 (huGdf5), a new member of the TGF-beta superfamily, has been determined through initial degenerate PCR and subsequent cloning and nucleotide sequencing of genomic DNA and cDNA encoding the precursor and flanking regions. The huGdf5 gene consists of only two coding exons. The protein is highly homologous to its murine equivalent, particularly the mature part which differs only by one amino acid. Expression in HuTK- cells using recombinant vaccinia virus revealed the expected processed dimeric mature protein. Antibodies against huGdf5 were raised in chicken.

Entities: Gene Species

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Year: 1994 PMID： 7980526 DOI： 10.1006/bbrc.1994.2508

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

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Cited

12 in total

1. Effects of GDF5 overexpression on embryonic rat dopaminergic neurones in vitro and in vivo.

Authors: David B O'Sullivan; Patrick T Harrison; Aideen M Sullivan
Journal: J Neural Transm (Vienna) Date: 2010-03-27 Impact factor: 3.575

Review 2. Nerve growth factor modulation of the cavernous nerve response to injury.

Authors: Anthony J Bella; Guiting Lin; Ching-Shwun Lin; Duane R Hickling; Christopher Morash; Tom F Lue
Journal: J Sex Med Date: 2009-03 Impact factor: 3.802

3. Ectopic induction of tendon and ligament in rats by growth and differentiation factors 5, 6, and 7, members of the TGF-beta gene family.

Authors: N M Wolfman; G Hattersley; K Cox; A J Celeste; R Nelson; N Yamaji; J L Dube; E DiBlasio-Smith; J Nove; J J Song; J M Wozney; V Rosen
Journal: J Clin Invest Date: 1997-07-15 Impact factor: 14.808

4. Isolation of a cDNA sequence of rabbit GDF5 (mature form) and pattern of its mRNA expression during periosteal chondrogenesis.

Authors: A Sanyal; G Sarkar; J S Fitzsimmons; S W O'Driscoll
Journal: Mol Biotechnol Date: 2000-11 Impact factor: 2.695

Cloning and expression of recombinant human growth/differentiation factor 5.

1. Effects of GDF5 overexpression on embryonic rat dopaminergic neurones in vitro and in vivo.

Review 2. Nerve growth factor modulation of the cavernous nerve response to injury.

3. Ectopic induction of tendon and ligament in rats by growth and differentiation factors 5, 6, and 7, members of the TGF-beta gene family.

4. Isolation of a cDNA sequence of rabbit GDF5 (mature form) and pattern of its mRNA expression during periosteal chondrogenesis.

5. Delayed fracture healing in growth differentiation factor 5-deficient mice: a pilot study.

6. Cloning and functional characterization of the 5'-flanking region of the human bone morphogenetic protein-2 gene.

7. Adenovirus-mediated expression of growth and differentiation factor-5 promotes chondrogenesis of adipose stem cells.

8. Receptor oligomerization and beyond: a case study in bone morphogenetic proteins.

9. Cloning of integral mature peptide gene of human GDF-5.

Review 10. The Good the Bad and the Ugly of Glycosaminoglycans in Tissue Engineering Applications.