Literature DB >> 7979980

Physiochemical and immunological characterization of hepatitis A virus nucleocapsids expressed in a vaccinia virus/T7/EMCV system.

N L Zhu1, G D Li, Y Wang.   

Abstract

Expression of the entire open reading frame of hepatitis A virus was achieved in a vaccinia virus/T7/EMCV hybrid system. The expressed antigens were characterized as particles with buoyant densities and sedimentation coefficients typical of empty capsids and pentamers of HAV. This was further confirmed by electron microscopy. All capsid proteins were determined as components of the particles which raised neutralizing antibodies in an immunized rabbit.

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Year:  1994        PMID： 7979980     DOI： 10.1007/BF01310029

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  9 in total

1.  Product review. New mammalian expression vectors.

Authors:  B Moss; O Elroy-Stein; T Mizukami; W A Alexander; T R Fuerst
Journal:  Nature       Date:  1990-11-01       Impact factor: 49.962

2.  Attenuation and cell culture adaptation of hepatitis A virus (HAV): a genetic analysis with HAV cDNA.

Authors:  J I Cohen; B Rosenblum; S M Feinstone; J Ticehurst; R H Purcell
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

3.  Systematic nomenclature of picornavirus proteins.

Authors:  R R Rueckert; E Wimmer
Journal:  J Virol       Date:  1984-06       Impact factor: 5.103

4.  New findings in live, attenuated hepatitis A vaccine development.

Authors:  P J Provost; R P Bishop; R J Gerety; M R Hilleman; W J McAleer; E M Scolnick; C E Stevens
Journal:  J Med Virol       Date:  1986-10       Impact factor: 2.327

5.  Morphogenesis of hepatitis A virus: isolation and characterization of subviral particles.

Authors:  D A Anderson; B C Ross
Journal:  J Virol       Date:  1990-11       Impact factor: 5.103

6.  Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.

Authors:  O Elroy-Stein; T R Fuerst; B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

7.  The hepatitis A virus polyprotein expressed by a recombinant vaccinia virus undergoes proteolytic processing and assembly into viruslike particles.

Authors:  P L Winokur; J H McLinden; J T Stapleton
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

8.  Identification and characterization of incomplete hepatitis A virus particles.

Authors:  F Ruchti; G Siegl; M Weitz
Journal:  J Gen Virol       Date:  1991-09       Impact factor: 3.891

9.  The 5'-terminal nucleotides of hepatitis A virus RNA, but not poliovirus RNA, are required for infectivity.

Authors:  S A Harmon; O C Richards; D F Summers; E Ehrenfeld
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103
  9 in total
  1 in total

1.  Processing of proteinase precursors and their effect on hepatitis A virus particle formation.

Authors:  C Probst; M Jecht; V Gauss-Müller
Journal:  J Virol       Date:  1998-10       Impact factor: 5.103
  1 in total

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