Literature DB >> 7978281

High-performance liquid chromatography method for the determination of protein-glutathione mixed disulfide.

K Kumari1, P Khanna, N H Ansari, S K Srivastava.   

Abstract

We have developed an efficient and sensitive method for the quantification of picomole quantities of protein-glutathione and protein-cysteine mixed disulfides in ocular lens. Performic acid oxidation releases glutathione and cysteine from the protein-mixed disulfides as glutathione sulfonic acid and cysteic acid, respectively. The sulfonic acids are derivatized with phenylisothiocyanate, and the phenylthiocarbamyl derivatives are separated by HPLC using a reversed-phase ODS column, equilibrated with 0.14 M sodium acetate, containing 0.1% triethylamine and 6% acetonitrile.

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Year:  1994        PMID： 7978281     DOI： 10.1006/abio.1994.1352

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Intrapolypeptide disulfides in human alphaA-crystallin and their effect on chaperone-like function.

Authors:  M Cherian-Shaw; J B Smith; X Y Jiang; E C Abraham
Journal:  Mol Cell Biochem       Date:  1999-09       Impact factor: 3.396

2.  Glutathionylation of lens proteins through the formation of thioether bond.

Authors:  Mikhail Linetsky; Roy D LeGrand
Journal:  Mol Cell Biochem       Date:  2005-04       Impact factor: 3.396

Review 3.  Measurement and identification of S-glutathiolated proteins.

Authors:  Bradford G Hill; Kota V Ramana; Jian Cai; Aruni Bhatnagar; Satish K Srivastava
Journal:  Methods Enzymol       Date:  2010       Impact factor: 1.600

4.  Novel cysteine-centered sulfur metabolic pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha.

Authors:  Min Jeong Sohn; Su Jin Yoo; Doo-Byoung Oh; Ohsuk Kwon; Sang Yup Lee; Andriy A Sibirny; Hyun Ah Kang
Journal:  PLoS One       Date:  2014-06-24       Impact factor: 3.240
  4 in total

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