Literature DB >> 7978279

Iodixanol: a nonionic iso-osmotic centrifugation medium for the formation of self-generated gradients.

T Ford1, J Graham, D Rickwood.   

Abstract

The physical and biological properties of Iodixanol, a new nonionic density gradient medium, are described in this paper. It is effectively a dimer of Nycodenz and it exhibits two significant advantages over previous iodinated density gradient media--its aqueous solutions are iso-osmotic up to a density of 1.32 g/ml and it is capable of forming self-generating gradients in 1 to 3 h. It has a very low toxicity toward biological material and enzyme assays can be carried out in its presence.

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Year:  1994        PMID: 7978279     DOI: 10.1006/abio.1994.1350

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  40 in total

1.  Tip-growing cells of the moss Ceratodon purpureus Are gravitropic in high-density media.

Authors:  Jochen Michael Schwuchow; Volker Dieter Kern; Fred David Sack
Journal:  Plant Physiol       Date:  2002-12       Impact factor: 8.340

2.  Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.

Authors:  Heinrich J G Matthies; Jessica L Moore; Christine Saunders; Dawn Signor Matthies; Lynne A Lapierre; James R Goldenring; Randy D Blakely; Aurelio Galli
Journal:  J Neurosci       Date:  2010-06-09       Impact factor: 6.167

3.  The matrix protein of Marburg virus is transported to the plasma membrane along cellular membranes: exploiting the retrograde late endosomal pathway.

Authors:  Larissa Kolesnikova; Sandra Bamberg; Beate Berghöfer; Stephan Becker
Journal:  J Virol       Date:  2004-03       Impact factor: 5.103

4.  Characterization of insulin-responsive GLUT4 storage vesicles isolated from 3T3-L1 adipocytes.

Authors:  M Hashiramoto; D E James
Journal:  Mol Cell Biol       Date:  2000-01       Impact factor: 4.272

5.  Separation of the principal HDL subclasses by iodixanol ultracentrifugation.

Authors:  Nicola L Harman; Bruce A Griffin; Ian G Davies
Journal:  J Lipid Res       Date:  2013-05-20       Impact factor: 5.922

6.  Mouse hepatitis virus replicase proteins associate with two distinct populations of intracellular membranes.

Authors:  A C Sims; J Ostermann; M R Denison
Journal:  J Virol       Date:  2000-06       Impact factor: 5.103

7.  A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes.

Authors:  Alin Rai; Haoyun Fang; Monique Fatmous; Bethany Claridge; Qi Hui Poh; Richard J Simpson; David W Greening
Journal:  Methods Mol Biol       Date:  2021

8.  Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos.

Authors:  Stefan Wiese; Thomas Herrmann; Carsten Drepper; Sibylle Jablonka; Natalia Funk; Alice Klausmeyer; Mary-Louise Rogers; Robert Rush; Michael Sendtner
Journal:  Nat Protoc       Date:  2009-12-17       Impact factor: 13.491

9.  Enrichment and analysis of secretory lysosomes from lymphocyte populations.

Authors:  Hendrik Schmidt; Christoph Gelhaus; Ralph Lucius; Melanie Nebendahl; Matthias Leippe; Ottmar Janssen
Journal:  BMC Immunol       Date:  2009-07-29       Impact factor: 3.615

10.  The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques.

Authors:  A Gregory Bruce; Angela M Bakke; Courtney A Gravett; Laura K DeMaster; Helle Bielefeldt-Ohmann; Kellie L Burnside; Timothy M Rose
Journal:  Virol J       Date:  2009-11-18       Impact factor: 4.099

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