| Literature DB >> 7975881 |
L Xu1, S Haga, S Imai, N H Sarkar.
Abstract
Plasmid subcloning by conventional techniques of full length exogenous mouse mammary viruses (MMTV) has not been realized because of the involvement of host-mediated structural changes in the viral gag gene. To circumvent this problem, an alternative subcloning method, excision of phagemid (pBluescript SK) from lambda ZAP II, was successfully used to subclone a novel exogenous MMTV (JYG-MMTV) provirus fragment containing an intact gag gene. Sequence analysis revealed that the LTR of this virus is significantly different from the LTR of C3H-MMTV in the U3 region.Entities:
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Year: 1994 PMID: 7975881 DOI: 10.1016/0168-1702(94)90053-1
Source DB: PubMed Journal: Virus Res ISSN: 0168-1702 Impact factor: 3.303