Effective induction of pregnancy block by electrical stimulation of the mouse accessory olfactory bulb coincident with prolactin surges.

Electrical stimulation (0.33 Hz, 0.2 mA) applied bilaterally to the accessory olfactory bulb (AOB) of newly mated female mice revealed that stimulation for 4 h was sufficient to produce pregnancy block providing stimulation is given for two separate 2-h periods coincident with prolactin surges. Stimulation for two 1-h periods coincident with prolactin surges or two 2-h periods between prolactin surges was without effect. These results indicate that electrical stimulation of the AOB can faithfully reproduce pheromone-induced pregnancy block and support the view that prolactin is the hormone mainly responsible for the olfactory block to pregnancy.