| Literature DB >> 796664 |
J D Beggs, M Guerineau, J F Atkins.
Abstract
The 2 micron circular DNA from S. cerevisiae has been cloned on bacteriophage lambda. The two forms of circular DNA which exist in equilibrium due to recombination between inverted repeat sequences were separated as stable clones, and a map of targets for restriction endonucleases EcoRI, HindIII and HpaI was constructed. The circular DNAs isolated from a particular oligomycin resistant strain and its parent oligomycin snesitive strain were compared by restriction endonuclease analysis, and no difference was detected. The potential uses of cloned 2 micron DNA in determining the possible biological role of these plasmids are considered.Entities:
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Year: 1976 PMID: 796664 DOI: 10.1007/bf00332903
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925